CS-2000i and CS-2100i System Hardware Overview Online Training

Identify Key Components Identify the function of each key component Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed theHardware Overview module. Once you have reviewed the content on this page, you may proceed to the assessment portion of the course. In this course, you have learned how to identify the key components and their functions of each area of the CS-2000i. Main Unit The Power Switch, Fuse Box and Power Cord are located on the left side of the Main Unit. The Trap Chamber is located behind a small door at the lower right on the left side of the Main Unit.  The Trap Chamber collects any moisture and prevents it from reaching the Compressor Unit. The Rinse Aspiration Connectors and Sensor, and the Waste Connectors and Float Sensor are located at the lower left of the left side. The 20 liter Rinse and Waste Containers are connected here. Filter No. 16, located underneath the left side of the Main Unit, prevents dust from entering the Main Unit. The Sampler, located on the front of Main Unit, automatically transports the samples to the Main Unit.  The Sampler is comprised of the Right Rack Pool where up to 5 sample racks are placed to be processed, the Aspiration Position where the samples are aspirated by the Sample Probe, and the Barcode Reader where sample barcodes are read. Racks move to the Left Rack Pool when sampling is completed. TheLight Shield Lid is located right above the Sampler on the Main Unit. It protects the Sample Arm and is locked down during analysis. The Sample Arm has a probe which is a piercer or needle for systems equipped with a Cap Piercer and a standard probe with a blunt tip for Non-Cap Piercer systems. Both are supplied with a crash sensor to detect obstructions. The probe aspirates patient samples, controls, diluents and calibrators and adds them to a cuvette. A pre-analytical check of the sample for Hemolysis, Icterus, and Lipemia (HIL) is performed by the HIL Detector, located behind the Reagent Table. If a sample is found to be positive for Hemolysis, Icterus, or Lipemia, it is noted on the Joblist. TheReagent Table holds reagents, calibrators, and controls and is located in the center of the Main Unit. Reagent Table A is the outer reagent rack on the Reagent Table. It contains 5 racks of which each holds up to 6 reagent vials.   Reagent Table B is the inner reagent racks. Each inner rack holds 2 reagent vials.   A total of 40 reagents can be placed on the Reagent Table.   The Reagent Table is maintained at approximately 10°C.    Both the inner and outer racks have 1 position for stirred reagents.   The Reagent Table utilizes an automatic barcode reading function. Each rack position has a barcode that is used to identify its position on the Reagent Table.   When a reagent, calibrator, or control is placed onboard the Reagent Table, the analyzer reads the rack position barcode, and the reagent vial barcode to positively identify the reagent and its position. The Reagent Arm is located at the rear of the Reagent Table. The Reagent Arm has a probe that aspirates reagents from the Reagent Table and adds them to the sample in the cuvette. The Reagent Probe has a crash sensor to prevent it from being damaged. The Dispensing Table surrounds the Reagent Table. The Dispensing Table holds cuvettes into which sample plasma is added from the primary sample tube or cup. It also moves cuvettes throughout the Main Unit for processing. Tray No. 48 is located underneath the Sampler on the front of the Main Unit. It collects condensation fluid in case of high room humidity. The STAT/Diluent Table is located at the front of the main unit. This table has 5 STAT sample holders and 5 diluent holders. The temperature is not controlled in this area and remains approximately room temperature. The cover of this table is monitored and locked for safety during sample processing. The STAT/Diluent LED indicates whether this area is accessible or not. Both the STAT positions and the Diluent positions utilize an automatic barcode reading function to positively identify each diluent or sample loaded into this area.  The Cuvette Hopper holds cuvettes used for sample processing. It is located on the upper right portion of the Main Unit. The Mechanical Stop Switch is located on the front of the Cuvette Hopper. The red Mechanical Stop Switch is used for immediate stoppage of all movement within the Main Unit and should be used only in an emergency situation. The green Start Button is located on the front of the Cuvette Hopper and is used to start sample processing The Alarm Indicator LED is located to the left of the Cuvette Hopper. This LED displays a color to indicate the status of the Main Unit. Flashing Green: Ready   Flashing Orange: Analyzing   Red: Error The Detector Unit, located behind the Reagent Table, contains 10 incubation wells and 10 detection wells.  The Detector Unit incubates and analyzes the mixture of sample and reagent in the cuvette. The Information Processing Unit (IPU) Connector is located on the right side of the Main Unit and connects the IPU to the Main Unit. The Halogen Lamp is located behind a door on the right side of the Main Unit. This lamp is the light source used for all assays performed on the Sysmex® CS-2000i. Light from the Halogen lamp is transmitted through filters on the Multi-wavelength Filter Wheel to create light at 340nm, 405 nm, 575nm, 660nm, and 800nm. All 5 wavelengths of light are carried to the Detector Unit where they are used to determine the test reactions for all Clotting, Chromogenic, and Immunologic Assays. Three wavelengths of this light (405nm, 575nm, 660nm) are transmitted  to the HIL Detector where it is used to determine if the sample is positive for Hemolysis, Icterus, and Lipemia The three Air Filters located on the back of the Main Unit block dust from entering the analyzer. Information Processing Unit: The Sysmex® CS-2000i is managed by using the Information Processing Unit or IPU for processing samples.  This area of the system contains the IPU, a 17 inch Touch Panel Display Screen, Mouse, Keyboard, and Printer.  The IPU uses Windows XP software with a CD burner. The IPU contains Settings information, Reagent information, Assay information as well as the ability to order assays, and view results. Pneumatic Unit The Pneumatic Unit supplies compressed air to the Main Unit to perform aspiration and dispensing for both the Sample and Reagent Arms. These pressures are monitored constantly by pressure sensors. The Pneumatic Unit has an Adjustment Knob on its front cover which is used to adjust the 0.22 MPa pressure for the Main Unit. Select Next to continue.   The Sysmex® CS-2000i/2100i system, a fully automated blood coagulation analyzer designed for the mid-volume laboratory: Measures parameters simultaneously using coagulation, chromogenic, and immunologic methods using multi-wavelength detection Performs pre-analytical check for interfering substances like hemolysis, icterus, and lipemia prior to any testing Includes Non-cap piercing model (Sysmex® CS-2000i) or Cap piercing model (Sysmex® CS-2100i) Select Next to continue.   Select the image areas to learn more about the units of the Sysmex® CS-2000i system.  When complete, select Next to continue. The Main Unit performs the sample analysis. Main Unit Learn about the multiple key components within the Main Unit. Slide NumberText BlocksCalloutsAudio ScriptImage File1Welcome to the Virtual Laboratory where we will look at the key components of the Main Unit of the Sysmex® CS-2000i system. Select Next to continue.  Note: If audio does not automatically start, select the play arrow in the top left to begin.Welcome to the Virtual Laboratory for the Main Unit of the Sysmex® CS-2000i Automated Blood coagulation Analyzer. Let’s look at the key components located in the Main Unit which work together to perform Sample Analysis.2Power Supply Power to the analyzer is controlled by the Fuse Box, Power Cord and Power Switch. Select Next to continue.  Select each number to review the corresponding text.CalloutsFuse BoxPower CordPower SwitchThe Fuse Box, Power Cord and Power Switch are located on the left side of the Main Unit. The Power Switch is used to turn the Main Unit on and off. 3Pressure Adjustment knobs and Trap Chamber 0.10MPa Adjustment Knob adjusts Main Unit pressure. 0.067 MPa Adjustment Knob adjusts Main Unit vacuum. Moisture is collected by the Trap chamber, preventing it from reaching the compressor unit. Select Next to continue. Select each number to review the corresponding text.CalloutsTrap Chamber0.067 MPa Adjustment Knob0.10MPa Adjustment KnobLocated behind a small door on the left side is a pressure adjustment knob, a vacuum adjustment knob and the Trap Chamber. The pressure adjustment knob is used to adjust the pressure of the Main Unit to 0.10MPa. The vacuum adjustment knob adjusts the vacuum of the Main Unit to -0.067 MPa. The Trap chamber collects any moisture and prevents it from reaching the compressor unit.4Components for the Rinse and Waste Functions: Rinse Aspiration Connectors and Sensors for the Rinse container Waste Connectors and Float Sensor for the Waste container Select Next to continue.  The Rinse Aspiration Connectors and Sensor for the Rinse container, and the Waste Connectors and Float Sensor for the Waste container are located at the lower left of the left side.5Air Filter Filter No. 16 A prevents dust from entering the interior of the Main Unit. Select Next to continue.  Filter 16A is located under the left side of the Main Unit. This filter prevents dust from entering the interior of the Main Unit6Sampler Samples are routed to the Main Unit by the Sampler. Select Next to continue.  The Sampler is located on the front of Main Unit and automatically transports the samples to the Main Unit.7Light Shield Lid: Protects the sample arm and probe Sample Probe aspirates and dispenses: Samples Buffers Controls Calibrators The Sample Arm can also have an optional cap piercer unit. Select Next to continue. Select each number to review the corresponding text.CalloutsSample ArmSample ProbeOn the Main Unit, above the Sampler is the Light Shield lid. The Light Shield lid protects the Sample Arm and probe. The Sample probe aspirates and dispenses samples, buffer, controls, and calibrators. The Sample probe is protected by a crash sensor and performs the sample tube fill check. An optional Cap piercer unit can allow the probe to aspirate samples through a closed tube.8The Reagent Table holds: Reagents Calibrators Controls Select Next to continue.  The Reagent table is located in the center of the Main Unit and holds reagents, calibrators, and controls.9The Dispensing Table: Holds Cuvettes Moves cuvettes Select Next to continue.  Select the number to review the corresponding text.CalloutsDispensing TableThe Dispensing Table is a circular table that surrounds the Reagent table. The Dispensing Table holds cuvettes into which sample plasma is added from the primary sample tube or cup. It also moves cuvettes throughout the Main Unit for processing10The HIL Detector checks for:    Hemolysis Icterus Lipemia Select Next to continue.The HIL Detector performs the pre-analytical check of the sample in the dispensing table for interfering substances. It is located behind the reagent table on the interior of the instrument.11The Detector Unit: is located behind the reagent table incubates and analyzed the cuvette mixture Select Next to continue. The Detector Unit is located behind the Reagent Table on the interior of the instrument. The Detector Unit incubates and analyzes the mixture of sample and reagent in the cuvette.12Cuvette Trash Tray located on the front right of the Sampler holds up to 500 used cuvettes Select Next to continue. The Cuvette Trash Tray is located on the front right of the Sampler. The tray holds a capacity of up to 500 used cuvettes and pulls out for easy access.13Tray No. 48 is located underneath the front of Main Unit collects condensation fluid Select Next to continue.Tray No. 48 is located underneath the front of the Main Unit. It collects condensation fluid in case of high room humidity.14STAT/Diluent Table Diluents, Buffer and STAT samples are placed in the STAT/Diluent Table. Select Next to continue.  The STAT /Diluent Table is located at the front of the Main Unit. Diluents, Buffer and STAT samples are placed here.15Cuvette Hopper The cuvettes used for sample processing are provided from the Cuvette Hopper. Select Next to continue.  The Cuvette Hopper holds up to 500 cuvettes for sample processing. It is located on the upper right portion of the Main Unit. A Start button which starts analysis and Mechanical Stop switch which stops all movement of the Main Unit, are located on the front of the Hopper.16Alarm Indicator LED Main Unit status is indicated by the LED on the Alarm indicator. Color Indicators Flashing Green: Ready Flashing Orange: Analyzing Red: Error Select Next to continue.  The Alarm indicator LED is located to the left of the Cuvette Hopper. This LED indicates status by color to indicate the status of the Main Unit.17IPU Connector The Information Processing Unit (IPU) Connection connects the Main Unit to the IPU. Select Next to continue. The right side of the Main Unit has the IPU connector which connects the IPU to the Main Unit.18Halogen Lamp is located behind a door on the right side of the main unit provides the light used to perform assay measurement. Select Next to continue.The Halogen lamp is located behind a door on the right side of the Main unit. This lamp is the light source used for all assays performed on the CS-2000i19Filter Wheel A multi-wavelength filter wheel changes light from the halogen lamp into 5 different wavelengths. Select Next to continue.  Select each number to review the corresponding text.CalloutsHalogen LampFilter WheelA multi-wavelength filter wheel. is located next to the Halogen Lamp. Light from the Halogen lamp is transmitted through filters on the Multi-wavelength wheel to create light at 5 different wavelengths of light.20Air Filters Dust is prevented from entering the analyzer by 3 filters. Filter No. 514 (2) Filter No. 513 (1) Select Next to continue.  Select each number to review the corresponding text.Callouts Filter No. 514 Filter No. 514Filter No. 513On the back of the Main Unit are three air filters. These filters are important in blocking dust from entering the analyzer. 21Congratulations.  You have learned how to identify the location of the key components on the Sysmex® CS-2000i Main Unit. When complete, select the X in the upper-right corner to close the window and continue.Congratulations. You now have learned how to identify the key components of the Sysmex CS-2000i Main Unit. The Sampler consists of four components: Right Rack Pool Aspiration Position Barcode Reader Left Rack Pool Sampler Area Learn about each component of the Sampler. Checklist TitleChecklist TypeChecklist ContentSamplerHTML Select each checkbox to learn more about the Sampler Area.   Right Rack PoolHTML Sample racks are loaded onto the Right Rack Pool for processing by the Main Unit.   The Sampler has continuous loading, and up to 50 samples can be loaded at one time.Aspiration PositionHTML The sample rack in the Right Rack Pool is transported automatically to the Aspiration Position, located in the center of the Sampler. Samples are aspirated by the Sample Probe in this area.Barcode ReaderHTML The Barcode Reader is positioned in the center of the sampler in front of the Aspiration Position. The Barcode Reader moves in front of the Sample Rack to read the barcode label of each sample tube in the rack.Left Rack PoolHTML The Sample Rack is transported automatically to the Left Rack Pool after it has been aspirated by the analyzer. When complete, select the X in the upper-right corner to close the window and continue. The Reagent Table holds: Reagents Calibrators Controls Cleaners Reagent Table Learn about the Reagent Table. Slide NumberText BlocksCalloutsAudio ScriptImage File1Welcome back to the Virtual Laboratory where you will learn about the Reagent Table of the Sysmex® CS-2000i analyzer. Select Next to continue.  Note: If audio does not automatically start, select the play arrow in the top left to begin.Welcome back to the Virtual Lab. In this visit, we will look at the structure of the Reagent Table.2Reagent Table Covers Reagent Table A: 5 Outer Reagent Racks accessed through Reagent Table Cover A Reagent Table B: 5 Inner Reagent Racks accessed through Reagent Table Cover B Select Next to continue.  Note:  Each cover has a LED light that indicates if it is accessible.Select each number to review the corresponding text.Callouts Reagent Table Cover A Reagent Table Cover BThe Reagent Table is a circular table containing 5 outer reagent racks and 5 inner reagent racks. The outer reagent racks are called Reagent table A. They are accessed through Reagent Table Cover A. The inner reagent racks are called Reagent table B. They are accessed through Reagent Table Cover B. For safety purposes, both Reagent Table covers are monitored and locked during sample processing.3Reagent Table  Reagent Table A: 5 Outer Reagent Racks hold 6 reagent vials (total 30) Reagent Table B: 5 Inner Reagent Racks hold 2 reagent vials (total 10) Maintained at 10°Celsius Select Next to continue.  Select each number to review the corresponding text.Callouts Reagent Table A Reagent Table BThe Reagent Table can hold up to 40 reagents and is maintained at approximately 10 degrees Celcius.4Racks One stir position per rack (indicated by a circle around the position number) Tilted reagent positions Adapter for different bottle sizes available Select Next to continue.  Select each number to review the corresponding text.CalloutsStir PositionAdapterBoth the inner and outer racks have one position for stirred reagents. The reagent positions are tilted to optimize efficiency of reagent use. Adaptors are used to place different size vials.5Barcode The barcode reader identifies: Racks Reagent position Reagents, calibrators, and controls Lots and bottle sizes Select Next to continue.  Select each number to review the corresponding text.CalloutsRack BarcodeRack Position BarcodeReagent BarcodeThe Reagent Table utilizes an automatic barcode reading function. Each rack position has a barcode that is used to identify the specific rack and the specific position in the rack. When a reagent, calibrator, or control is placed onboard the Reagent Table, the analyzer reads the rack position barcode, and the reagent vial barcode to positively identify the reagent and its position.6Reagent Arm The Reagent Arm Probe aspirates reagents and adds them to the sample in the cuvette. The Crash Sensor protects the Reagent Probe from being damaged. Select Next to continue.  Select each number to review the corresponding text.Callouts Reagent Arm Reagent Probe The Reagent Arm is located behind a cover at the rear of the Reagent Table. The reagent arm has a probe that aspirates reagents from the Reagent Table and adds them to the sample in the cuvette. The reagent arm has a crash sensor. The crash sensor prevents the Reagent probe from crashing into an object such as a vial cap. The crash sensor will sense the obstruction and tell the probe to move away from it, thus preventing the probe from being bent or broken.7Congratulations.  You have learned about the Reagent Table.When complete, select the X in the upper-right corner to close the window and continue.Congratulations! Now that you learned about the Reagent Table, let’s next look at the Light Shield Lid area. The Light Shield Lid protects the Sample Arm which aspirates and dispenses calibrators, controls, diluents, and samples. Light Shield Lid Area Learn more about Cap Piercer and Non-Cap Piercer The non-cap piercing model (Sysmex® CS-2000i) has a blunt ended probe to aspirate from a sample tube with the cap removed. The non-cap piercing probe has a crash sensor. The crash sensor on the Sysmex® CS-2000i sample probe prevents it from being damaged by detecting an obstruction and causing the probe to move away from the obstruction. The cap piercing model (Sysmex® CS-2100i, pictured) has a beveled edged needle that will pierce through a sample tube cap and includes a crash sensor. The crash sensor on the Sysmex® CS-2100i sample probe prevents it from being damaged by detecting an obstruction and causing the probe to move away from the obstruction. The crash sensor, however, does not prevent the probe from piercing a sample tube cap. When complete, select the X in the upper-right corner to close the window and continue.   Managing pre-analytical variables is critical in obtaining the most accurate results in hemostasis testing. HIL Detector performs pre-analytical check of  the sample for: Hemolysis Icterus Lipemia HIL Detector Learn about the HIL Detector Plasma from the primary sample tube is aspirated by the Sample probe and placed into a cuvette on the Dispensing Table. The Dispensing Table rotates the cuvette to the HIL Detector. At the HIL Detector, light is transmitted at 405nm, 575nm, and 660nm through the cuvette and the absorbance is measured. The Sysmex® CS-2000i uses the absorbance readings of these three wavelengths to determine if the sample is Hemolysed, Icteric, or Lipemic. The HIL (Hemolysis, Icterus, and Lipemia) check is performed before sample measurement is started.  If a sample is found to be positive for Hemolysis, Icterus, or Lipemia, it is noted on the Joblist. Select play to begin the video. When complete, select the X in the upper-right corner to close the window and continue. The STAT/Diluent Table contains positions for STAT samples, buffers and diluents which remain at room temperature. For safety purposes, it is monitored and locked during sample processing. The STAT/Diluent LED indicates whether this area is accessible or not. Both the STAT positions and the Diluent positions utilize an automatic barcode reading function to identify each diluent or sample loaded into this area. STAT/Diluent Table Learn about the STAT/Diluent Table. Base ImageHotspotsText BlocksImage FileDiluent Holders The 5 Diluent Holders hold diluents and buffer needed for testing on the Main Unit. Adaptors for different vial sizes are available. This area is at room temperature.STAT Sample Holders The STAT/Diluent Table has 5 STAT Sample Holders. STAT samples added here will be processed ahead of samples already loaded to the Sampler. STAT samples can be processed faster when loaded at this location. The Cuvette Hopper Hold approximately 500 cuvettes Supplies cuvettes to Main Unit via Cuvette Feeder and Supply Catcher You will learn more about the Cuvette Hopper in the Maintenance Module. Note: Green Start Button begins Sample Processing. Red Mechanical Stop Switch stops all movement in the Main Unit in emergency. Cuvette Feeder & Supply Catcher Learn more about Cuvette feeder and the Supply Catcher. The Cuvette Feeder rotates the cuvettes into position for the Supply Catcher. The Supply Catcher is a rotary arm that picks up the cuvettes from the Feeder Unit and positions them in the Dispensing Table. The Supply Catcher also retrieves used cuvettes from the Dispensing Table and discards them in the Cuvette Trash Tray.      When complete, select the X in the upper-right corner to close the window and continue.        The Detector Unit contains: 10 Incubation Wells 10 Detection Wells Select Next to continue. 340nm 405nm 575nm 660nm 800nm Select Next to continue. The Information Processing Unit (IPU) manages: Order Assays and View Results Reagent and Multi Lot management Calibration QC Assay Settings Select Next to continue The Pneumatic Unit: Supplies compressed air Monitored by pressure sensors Controlled by 0.22 MPa pressure adjustment knob Select Next to continue.