AUTOPAK 300 Clinical Analyzer Instrument Overview Online Training

Welcome to the AUTOPAK 300 Clinical Chemistry Analyzer Overview online training. The AUTOPAK 300  is a reliable in-vitro diagnostic, bench-top, analyzer for automatic testing of routine clinical chemistry tests and electrolytes. This course is designed to introduce you to have an overview of AUTOPAK 300, its main modules and  components, their locations and functions, which will help you operate and maintain the system with maximum efficiency.​ Click Next to go to Course Objectives. Upon completion of this online training, you will be able to: Identify different instrument modules, components, their locations and functions   Understand the features and specifications of the analyzer. Learn more about various associated components that work with the major modules in AUTOPAK 300. Click Next to go to the detailed description of AUTOPAK 300. Have an overview of AUTOPAK 300 & AUTOPAK 300i, their differences etc.  In this AUTOPAK 300 Overview Online Training, you have learnt about: - AUTOPAK 300 and AUTOPAK 300i models - Their features and various modules - Location and function of various components - AUTOPAK 300 software - Various User Interface screens - Important Buttons on the Monitor Screen Now it is time for a Final Test of what you have learnt. Click Next to go for the Final Test.   AUTOPAK 300 is a reliable in-vitro diagnostic analyzer for automatic testing of routine clinical chemistry tests and electrolytes. It has two models, namely: AUTOPAK 300 - only Chemistry, no Electrolytes, Max. throughput = 300 tests per hour. AUTOPAK 300i - Chemistry & Electrolytes, Max. throughput = 480 tests per hour. The complete AUTOPAK 300 system comprises of - the Basic Analyzer  - one Desktop Computer  - Containers for Water, Waste and Cleaning Solution To have a view of the instrument externally from different angles, please click on the Links below. Click Next to go to Instrument Features. Front View Front View Side View Side View The upper switch is for Mains ON/OFF. The lower switch is for Reagrnt Cooling ON/OFF. Rear View Rear View AUTOPAK 300 has the following modules:         Sample Tray                                            Reagent Tray   Pipettor Arm                                             Photometer   Bar Code Reader                                     ISE (optional)   Desktop Computer                                   APK300 Software   Click the Link below to see the Architecture of it.           Click Next to go to the Sample Area. Instrument Architecture Instrument Architecture AUTOPAK 300 software is installed in the Desktop Computer that comes with the Analyzer. Details of the computer are as: Windows ™ 8 or higher computer, 4 Gbytes RAM, 500 Gbytes HDD, DVD-ROM drive, Min. 1024x768 resolution SVGA 15” or bigger monitor, Keyboard, Mouse, 2 x USB ports link with the analyzer,1 RS-232 port or 1 USB/RS-232 converter for link the LIS, USB port for printer. Click below Links to learn more about the User Interface of AUTOPAK 300. Click Next to learn more about the various features in the instrument software.   Main Page Main Page Important Buttons Important Buttons Sample Area is made up of:         SampleTray - located around the Reagent Tray and is driven by seperate Stepper Motors, capable of rotation         in both clockwise and counter-clockwise directions. It contains 5 sample racks, 19 sample per rack. ~ 95 (5 rack x 19 pos.)          Optical Sensor -  for Home or reference, works in association with Stepper Motor. Slotted-Wheel Set - provides movement detection in steps.   Barcode Reader - mounted on the outer border of sample tray to detect and recognize samples and reagents.             Click on the Link below to learn more about Sample Tray. Click Next to go to the Reagent Area. Sample Rack Sample Rack Sample Tray contains 5 sample racks, 19 sample per rack. ~ 95 (5 rack x 19 positions), Primary tubes of length up to 100 mm and 13 - 16 mm diameter can be used. Pediatric vial can also be used here.         Sample Volume: 2 to 100 μL/test ( in increments of 1 μL). The Reagent Area is made up of: Reagent Tray - driven by stepper motors, capable of rotation in both clockwise and counter-clockwise directions. Peltier Elements - for maintaining the temperature at 8 °C ± 1 °C. Electronic Control System - to ensure proper temperature for storing the reagents in the instrument.   Removable Reagent Tray can hold  72 single reagents. Up to 3 different reagents per method with multiple vials on board. Reagent volume: 1 to 3 reagents, 5 to 480 μL/test each (in increments of 1 μL) Reagent Container capacities: 25, 45 and 70 mL                    Click on the Links below to learn more about Reagent Area                Click Next to go to the Reaction Area. Reagent Tray Reagent Tray               Removable Reagent Tray having spilit positions to hold up to 72 single reagents.         Up to 3 different reagents/test with multiple vials on board.         Refrigerated compartment at 8 °C ± 1 °C.         Reagent volume: 1 to 3 reagents, 5 to 480 μL/test each (in increments of 1 μL)   Reagent Containers Reagent Containers Reagent bottles capacities:25, 45, 70 mL Reagents: 1 to 3 reagents, 5 to 480 μL/test each (in increments of 1 μL)   Reaction Tray Unit has: - a Stepper Motor,  - Optical Switches for home and positions,  - Heater for incubation at 37 degree C. - Photometer and  - Cuvette Wash Unit. Click on the Links below to learn more about Reaction Tray Click Next to go to the Pipettor Arm. Reaction Cuvette Reaction Cuvette Reaction cuvette: Total 80 re-usable plastic cuvettes, divided into 16 strips each having 5 cuvettes Optical length: 6 mm Reaction volume : 180 – 500 μL Warm air incubator: 37ºC. Reaction time: 0 to 10 min. Stirring: After dispensing each reagent by the Pipettor Probe   Wash Station Wash Station Washing station : 8 steps by 8 probes. Water consumption: 1.5 L / hour (DIW)   Cuvette Washing Cuvette Washing AUTOPAK 300 has one Pipetting Arm for both sample and reagent delivery. Its various components are: Probe Assembly having two stepper motors for vertical and horizontal movements. Two Optical Switches for sensing home position and verification for each movement working similar to sample tray. Capacitive Level Detect Circuitry. Probe Pre-heater for thermostatize reagent 0.5 ºC above selected reaction chamber temperature. Collision Detector for prevent accidental probe damage and mixer system. Reaction Mixer. Both Internal and external washing of the probe are performed. Click on the Links below to learn more about associated components of Pipetting Arm. Click Next to go to the Photometer.   Diluter Diluter The diluter, equipped with 500 uL syringe - aspirates and dispenses reagent and sample consecutively. Air gaps separate liquids to prevent early mixing and contamination.   Clot Detector Clot Detector     The clot detector is a module to detect and warn about potential clogs or blood clots during a run. The pressure in the sample tube is measured and compared with the normal state during aspiration. The photometer is provided with 12 interference filters mounted in a rotating filter wheel. Light beam from a Tungsten Halogen source passes through the selected filter, traverses the reaction cuvette. After absorption by the cuvette, the remaining part of light falls onto the detector to measure density of the reaction in the cuvette.   Photometric Range : - 0.1 to 3.6 A. Measuring wavelengths : 12 Optical Filters from 340 nm to 750 nm. Photometry: Single or Double-wavelength simulteneous reading.   Click the link below to see the details of the 12 optical filters. Click Next to go to the BCR (Bar Code Reader). Optical Filters Optical Filters Measuring wavelengths : 340, 380, 405, 450, 490, 505, 550, 590, 620, 650, 700, 750 nm. Internal Bar Code Reader is located at the back side of the Reagent/Sample Area. It detects and recognizes, - Samples,  - Controls,  - Calibrators or Standard solutions, and  - Reagents. Click Next to go to ISE Module.   The ISE (Ion Selective Electrode) module measures the concentration of Li+, Na+, K+, and Cl- in serum, plasma and diluted urine. ISE Module includes ion-selective electrodes and three peristaltic pumps. An integral sample entry port is positioned on top of the ISE Module, where the instrument's probe dispenses the sample. This compact design allows for small sample size and fast operation. Click Next to go to a quick knowledge check.. ISE Schematic ISE Schematic AUTOPAK 300 instrument has the below mentioned features: System: Full random access or batch mode operation with STAT sample priority function.   Throughput: Up to 300 photometric tests/hour and/or up to 480 tests/hour with optional ISE unit.   Methodology: Multiple methodology options - End point, Kinetic, Coagulation, Calculated,Turbidimetry, Enzymes and Drugs of abuse.   Calibration: Linear, Multi-linear, Sigmoidal, Logit-log 4, Logit-log 5, Spline: using up to 10 standards.   Quality Control: Levy-Jennings, Westgard rules and Twin Youden plots.   Automatic Sample Dilution: Pre-dilution and Post-dilution on abnormal levels, excessive Substrate consumption and/or lack of linearity. Automated Reflex Testing. Click Next to go to various modules of AUTOPAK 300. The Data tab in the User Interface have the features as:  - In service mode you can change passwords. - Import: Methods, QC, Calibrators, Translator, Units. - Service Backup can be done of entire Database or only last 7 days, with or w/out translator. - Shrink database to smaller size that will speed up the PC. - Use Reconnect if you lost communication with the instrument or you want to store new data to the CPU (e.g. mechanical calibration). Click Next to go to the next page.   Use Reports tab to view and take print out of the latest, - Photometer Calibration - Mechanical Calibrations  etc. Historic Versions includes current and previous versions of software. Click Next to go to the next page. In addition to the numerous other important functions, the Maintenance tab has following features: - Scheduler allows to track daily routine and maintenance required by the system - System Alerts preview alerts of open covers or missing solutions or full waste - System Log is a tracker of each step taken by the operator. Includes communication and errors. - Electronic Status presents current status of all electronics in the system. Click the Link below to learn more about Maintenance options. Click Next to go to the next page Maintenance Maintenance Slide NumberText BlocksCalloutsAudio ScriptImage File1Hydraulics: System Flush is a useful function used mostly in case of flushing but also refilling system. It is recommended to use it each time you perform any hydraulics related maintenance (e.g. Washer Volume Calibration)Enter your script here.2Motors: Press the Turn Off button to turn off all the motors in the instrument. Normally motors are energized so the modules cannot move freely. Important note: do not forget to initialize the instrument after using this function in order to find home positions of all modules.  Enter your script here.3Movements: Movements Menu allows to perform desired actions like movements, aspirations, dilutions as well as photometer readings. It might be helpful in diagnosing and checking particular functions of the instrument.Enter your script here.4Versions:     Versions Menu includes such information like Serial Numbers of components/modules and their versions gathered in one chart. This information is downloaded directly from the CPU board.  Enter your script here.5Wear: Wear Menu allows you to preview current usage and limits of several consumables. Each can be reset after replacing during periodical maintenanceEnter your script here.6Click here to edit text.Parameters: Menu Parameters collects many system parameters which can be set and adjusted according to individual requirements.Enter your script here.7General: In General Parameters you can change language, COM port, warnings, clot behavior etc.Enter your script here.8BCR: In BCR Parameters you can set barcodes on samples, reagents and sectors.Enter your script here.9LIMS: In LIMS Parameters you can enable and set LIMS connection.Enter your script here.10Equipment: In Equipment Parameters you can choose the instrument type.Enter your script here.11Use: In Use Parameters you can the blank limits as well as type of tubes used in lab.Enter your script here. System software has different levels of access depending of the type of user: Normal User is the default condition. In this case, only system operation is possible. Power User can define control sets and calibration sets. Supervisor User can have  increased level of access. Service User is for Service Engineers. One can login as Power User, Supervisor or Service by providing a corresponding Password. Log Out option will return to operator level. Main menu allows the modification of passwords. Click Next to go to the Data option. Below are a few Quick Keys with their descriptions which are used frquently. Click Next to go to learn about various access levels.