Atellica® NEPH 630 System System Overview Online Training

Welcome to the Atellica™ NEPH 630 System* Overview Online training. The Atellica NEPH 630 System is used for the quantitative determination of proteins in serum, urine, and cerebral spinal fluid (CSF). Some system features are: Walk away capability 45 samples can be loaded simultaneously Preparation of dilutions for samples, standards and controls Atellica is a trademark of Siemens Healthcare Diagnostics Inc., or its affiliates. *Not available for sale in the U.S. Product availability may vary from country to country and is subject to varying regulatory requirements.   Upon successful completion of this course, you will be able to:   Identify the key hardware components and describe their functions Navigate key software screens and describe their function Congratulations. You have completed the Atellica NEPH 630 System Overview Online Training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Identify the key hardware components and describe their function External components: Left and right covers - allows access to internal components LEDs - indicates the status of the system by the color of the illuminated LED Power module - contains fuses, main power switch and power supply socket Liquid containers and waste - supplies diluent, buffer, and purified water and collects waste Peripheral devices - computer, keyboard and mouse and optional printer Internal Components: Sample rotor - samples and standards are loaded here Syringe - supplies the probe with diluent and purified water Reagent rotor - reagents and controls are stored here Probe Prepares dilutions of samples, controls and standards Dispenses dilutions, reagents, and buffer into cuvettes Mixes liquids Checks for dilution strips and performs liquid level sensing Wet station Cleans the probe internally and externally Contains buffer reservoir with buffer necessary for certain assays Dilution station - samples, controls and standards are mixed with diluent in the dilution strips Cuvette rotor - Dilutions, reagents and buffer are dispensed into the cuvette segments  Navigate key software screens and describe their function Areas of the software screen Title bar - displays title of active dialog Navigation bar - select the routine buttons to access Status, Lab Journal, Loading Calibration, System, QC and Help dialogs Status bar - contains status buttons for various parts of the system. Background color of each button represents the status Blue - System is operating. Yellow - System is warming up or jobs cannot be completely processed Red - System is not operating Status line - displays date, time, actions currently performed and last job Dialog - displays details of the routine selected Action bar - displays action buttons appropriate to the open dialog   Dialogs Status- manages status of sample rotor, reagent rotor, cuvettes, dilution cups, reference curves, controls, liquids and waste Lab Journal - manages patient and control results Loading - manages loading samples, standards, reagents, controls, cuvette segments and dilution strips Calibration - manages calibration and displays reference curves System - manages maintenance, access, hardware, software and resetting of the analyzer QC - active when QC software has been loaded Status bar  Buttons indicate status of sample rotor, reagent rotor, cuvettes, dilution cups, reference curves, controls, liquids and waste Buttons can be blue, yellow or red depending on the current status   Components accessed through left cover: Sample rotor Syringe Components accessed through right cover: Reagent rotor Probe and wet station Dilution station Cuvette rotor   Note: Do not open the covers without access being requested via the software.     Internal Components Learn about internal hardware components. Base ImageHotspotsText BlocksImage FileSample rotor - samples and standards are loaded hereSyringe - supplies the probe with diluent and purified waterReagent rotor - reagents and controls are stored here. It is cooled to 8 degrees Celsius and covered to ensure constant temperatureDilution station - samples, controls and standards are diluted in the dilution strips that are located hereProbe and wet station - the probe samples patients, controls and standards. The wet station cleans the probe and contains the buffer reservoirCuvette rotor - contains cuvette segments. Dilutions, reagents and buffer are dispensed into cuvettes for measurement. It is heated to 37 degrees Celsius and covered to ensure constant temperature  Left cover (pictured opened)Right cover (pictured opened) Operator selects routines to operate the system Selecting a routine button opens a dialog for that routine Action buttons for the routine are active appropriate to the open dialog   Basic Areas Learn about the areas of the software screen. Base ImageHotspotsText BlocksImage FileThe navigation bar is used to access the routines of the system such as Status, Lab journal, Loading, Calibration, System, QC and Help. QC is active if the QC software has been installed.The title bar displays the title of the active dialog which corresponds to the selected routine and action.The status bar contains status buttons which display the status of various parts of the system. The status buttons are displayed to the right of most dialogs.The status line displays date, time and action currently performed by the system, and details of the job last finished by the system.The dialog displayed is dependent on the active routine in the navigation bar. Displayed in this example is the Lab journal dialog.The action bar displays the action buttons appropriate to the dialog displayed. White icons indicate the corresponding routine or action is not available. Holds three sample rotor segments with a maximum capacity of 45 samples Each sample rotor segment is fitted with adapters to hold different kinds of containers Remove all caps and stoppers before loading samples or standards into the appropriate segment type Sample Rotor Segments Learn about sample rotor segments. Tab TitleTextSample Segment Holds up to 15 tubes White adapters hold sample tubes of 11 to 13 mm outer diameter Black adapters hold sample tubes of 13 to 16 mm outer diameter Standard Segment Holds up to 6 standard vials used to perform calibrations Only positions 3,5,7,9,11 and 13 must be used Eppendorf Cup Segment Fitted with adapters to hold up to 15 Eppendorf cups. Cup Behind Tube Segment Fitted with two rows of adapters to hold up to 7 sample tubes and 7 cups Lower row holds empty sample tubes with barcodes that identify the sample behind Upper row holds the cups with the sample material Holds 15 segments for reagents and controls Has a cover with an access lid Both reagent and control vials must be fitted with evaporation caps before loading Segments and evaporation caps are color coded   Reagent Rotor Learn about reagent rotor. Tab TitleTextEvaporation Caps and Segments Reagent and control vials must be fitted with caps before placing into the appropriate rotor segment White caps are placed on reagent vials and inserted into segments with white grips (pictured) Yellow caps are placed on control vials and inserted into segments with yellow grips (not pictured) Loading and Unloading Segments Open the access lid of the reagent rotor cover to load or unload reagent and control segments.    Loading and Unloading Vials Open the access lid of the reagent rotor cover to load or unload individual reagent or control vials. Holds 10 cuvette segments Heated to 37 degrees Celsius Dilutions, reagents and buffer are dispensed into cuvettes for assay Cuvette Rotor Learn about the cuvette rotor. Tab TitleTextCuvette Segments The cuvette rotor holds10 cuvette segments Each cuvette segment contains 9 cuvettes Installing Cuvette Segments Install a cuvette segment by handling it by the grip without touching the surfaces of the cuvette. Used to prepare dilutions of samples, controls and standards Consists of a dilution frame into which dilution strips are loaded   Dilution Station Learn about the dilution station. Tab TitleTextDilution Strip Each dilution strip consists of 6 dilution cups. Dilution Frame Dilution strips are inserted into the dilution frame which holds a maximum of 16 strips. Main components: Left and right covers LEDs Power module Liquid containers and waste Peripheral devices - computer, keyboard, mouse and printer (optional) External Hardware Components Learn about external hardware components. Base ImageHotspotsText BlocksImage FileRight cover - allows access to the reagent rotor, wet station, probe, dilution station and cuvette rotor.Left cover - allows access to the sample rotor and syringe.Peripheral devices - connected on the right side of the system. These include the computer, monitor, keyboard, mouse and printer (optional).Power module - located on the right side of the system. It contains 2 fuses, main power switch and power supply socket.Liquid containers and waste - located on left side of the system. They supply diluent, buffer and purified water to the system and collect waste.LEDs - They indicate the system status by the color of the illuminated LED. Green means no problems, yellow means initializing or can continue but cannot complete processing and red means the system cannot continue processing. Probe: Pipettes samples, controls and standards into dilution cups Pipettes reagents, buffer and dilutions into the cuvettes Mixes liquids in the dilution cups and cuvettes Checks for dilution strips Determines level of liquid in samples, standards, reagents, controls, dilution cups, and cuvettes Wet station consists of three areas: Buffer reservoir which contains the buffer necessary for certain assays Dispensing area in which the probe is cleaned internally Fountain area in which the probe is cleaned externally   Displays to the right of most dialogs Select the button to investigate further Color coded Blue - the system is operating. All requested jobs can be processed Yellow - the requested jobs cannot complete processing. If the user does not resolve the problem, the status button may change to red soon. The button is also yellow when the system is warming up Red - the jobs cannot continue processing. The system is not operating   Status Bar Learn about the status bar. Base ImageHotspotsText BlocksImage FileWalk-away time or Unfinished jobs  Estimated walk-away time is displayed or the number of unfinished jobsReagents & Controls Status of the level loaded in the reagent rotor  Cuv. seg & dil strips Status of the cuvette segments and dilution strips  Reference Curves & Control Results Yellow button indicates the system cannot complete processing.Information Includes temperatures, covers and access lid statuses and levels of system liquids and waste  Samples & Standards Status of the level loaded in the sample rotor   Select a routine button to open the corresponding dialogs: Status Lab journal Loading Calibration System Dialogs Learn about the dialogs. Tab TitleTextStatus Dialog Displays information for samples, standards, cuvettes, dilution cups, reagents, reference curves, controls, liquids and waste Background colors are: blue (operating), yellow (warming up or jobs cannot complete processing) and red (not operating) Lab Journal Dialog Samples and controls are displayed in a table Patient details includes sample ID, demographics, results and flags Control details includes control ID, results and flags Actions such as repeat and release result are requested here Loading Dialog Displays information for samples, standards, reagents, controls, cuvette segments and dilution strips on the system Request access to the sample and reagent rotors, cuvette segments, and dilution tray from this dialog Calibration Dialog Used to view, manage and check reference curves Actions such as measure, repeat and show curve are requested here System Dialog Used to perform maintenance, request access to hardware, check and initialize hardware, update and backup software and reset the analyzer Actions such as rinse the system and replace system liquids are requested here