Preparation of the Most Commonly Used Reagents Workflow on the Atellica® Solution

Continue Continue Continue Main Scene Preparation of the Most Commonly Used Reagents Workflow on the Atellica® Solution Day in the Life of the Lab Series Look for more content in our Day in the Life of the Lab Series. Welcome Welcome to the Preparation of the Most Commonly Used Reagents Workflow on the Atellica® Solution. A part of our Day in the Life of the Lab Series. This training is specific to Siemens Healthineers Assays and their methods. Blank Hi, it’s me Sandy. I am an experienced user and I have responsibility for training others in my lab on the Atellica Solution. One of the new hires recently asked, we have everything loaded on our system, and we are ready to go, but how do we do reagent preparation for some of our Siemens Healthineers Assays? There are a couple of examples of how we recently prepared and handled reagents and calibrators in my lab using official documentation, the instruction for use or IFU, that I would like to share. As I have mentioned before, I like to make sure everyone uses the same steps. How We Get Started Gather source information: - Assay Chart - Instructions for Use (IFU) Both the Assay Charts and IFU are available on Document Library. H To get started we gather the source information about the few Siemens Healthineers CH and IM assays in your labs menu that need special handling. This source information resides in two documents. One being the assay chart, specific to either CH or IM, that contains the necessary test codes. And then the IFU. The IFU is an important piece of this workflow. It gives us information on the intended usage of the assay, a summary of the assay, its principal usage, and directions on how to prepare the reagent. The details about special handling can be found in both of these. Your Choice Please click on the buttons below to select the Siemens Healthineers Assays for special preparation. Vitamin B12 Folate P5P Method Troponin Blank It’s your choice. Please click on the buttons below to select the Siemens Healthineers Assays for special preparation that you are interested in learning more about and I will explain the workflow I followed. Please Note: Besides the materials that have some special handling, if you are using other reagents and materials always follow manufacturer instructions. Are you ready for a knowledge check? Blank Besides the Siemens Healthineers materials that have some special handling, if you are using other reagents and materials, always follow the manufacturer’s instructions. Are you ready for a knowledge check? Knowledge Check You have just learned about Preparation of Most Commonly Used Reagents Workflow on the Atellica® Solution. Select Start to test your knowledge of the presented content. Start Knowledge Check Select the best answer. The Instructions For Use contains specific instructions on how to prepare the reagent. Question 1 of 1 Which is TRUE about the Instructions For Use? The Instructions For Use can be found on PEPconnect. The Instructions For Use provide a compiled list of all the CH and IM Assays and includes key information on calibrator, reagent, and diluent. Multiple Choice Incorrect Incorrect The CH or IM assay charts include a compiled list of all the assays Incorrect The Instructions For Use can be found on document library. Knowledge Check Completion Review Retry Retry Continue Continue You have just completed the Knowledge Check. Select Review to assess how your responses compare to the correct answers. Select Retry to test your knowledge again or select Continue to advance through the course. Knowledge Check Completion Refer to Online Help and PEPconnect for more information! Blank Thank you for taking the time to learn more about the preparation of the most commonly used reagents on the Atellica Solution. For more information you can search by specific keywords on Online Help, or check out PEPconnect, where you have access to additional job aids and videos. I look forward to spending another day in the life of the lab with you very soon and sharing more information about the Atellica Solution. Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. © Siemens Healthcare GmbH 2023 Siemens Healthineers Headquarters\Siemens Healthcare GmbH\Henkestr. 127\ 91052 Erlangen, Germany\Telephone: +49 9131 84-0\siemens-healthineers.com Disclaimer Exit A part of the Day in the Life of the Lab Series You have completed Preparation of the Most Commonly Used Reagents Workflow on the Atellica® Solution Completion Vitamin B12 Vitamin B12 How my lab follows Siemens Healthineers methods to prepare this Assay. Important: Careful preparation of the DTT Releasing Agent is required. Blank So, in my lab we recently encountered this first exception where we needed to prepare the ancillary reagent for Vitamin B12 or its official name, Atellica IM VB12 DTT Releasing Agent. It isn’t hard to do. We reviewed our documentation and I have some pictures to share of how we did it. Make sure to follow your labs specific requirements for pipetting. Add 300 µL DTT to 12.0 mL Releasing Agent in a test tube using a volumetric pipette. Note: Select the X at the top of the page to return back to the course. Blank To prepare this reagent in our lab you will see that we begin by adding 300 microliter DTT to 12.0 milliliter Releasing Agent in a test tube using a volumetric pipette. B2_002 We then cover our test tube with laboratory film or what I prefer using is a cap. I feel it is more accurate and there is less spillage if you are trying to do this quickly. In your lab you can use whatever container you have available and of course make sure to follow your labs specific requirements for pipetting. B2_001 Then we mix several times by inverting the test tube. B2_003 Then we carefully pour the contents of the test tube into the ancillary reagent pack. B2_006 Then we finish by putting a pack seal on the ancillary pack and pressing firmly. It is really convenient that these packs come with their own seal. B2_008 Folate Folate How my lab follows Siemens Healthineers methods to prepare this Assay. Blank Next I want to take a look at the preparation of Folate. Folate or Folic acid is a very important test for use in the diagnosis of anemia, and my lab runs these tests on our IM analyzer. Again, I have some photos on my phone to show you the process we follow in my lab. Always use care when transferring materials from one vial to another. Note: Select the X at the top of the page to return back to the course. Blank We begin by transferring the contents of 1 vial of Releasing Agent into 1 vial of DTT. I like to use a pipette to do the transfer. Folate_001 Then I put the cap on the vial Folate_004 and invert it several times to mix. Folate_005 I then transfer the entire contents of the vial into the ancillary reagent pack. Folate_008 I put a pack seal on the reagent pack and make sure that it is centered, and I make sure to press firmly. Folate_009 P5P Method P5P Assays ALTPLc and ASTPLc Both require two reagents: Reagent 1 (R1) - needs preparation Reagent 2 (R2) - ready to use . Blank The ALTPLc and ASTPLc assays, both use the P5P method. Both assays require the use of 2 reagent packs. Reagent 2 is ready to use, while Reagent 1 requires preparation. The Reagent 1 preparation steps are the same for both assays. Let me share how we recently prepared the R1 Reagent in our lab. Preparing the Reagents The Atellica CH P5P_L reagent is supplied ready for use, it may be added directly into the Atellica CH ASTPLc R1 reagent: 1. Carefully open one vial of the Atellica CH P5P_L and well 1 of the Atellica CH ASTPLc P1 pack. 2. The P5P_L may be poured or pipetted into well 1 of the ASTPLc P1 pack. - While holding the P1 pack steady on the bench, carefully pour the contents of the P5P_L vial into well 1 of the ASTPLc P1 pack. Note 300 μL of P5P_L will be added to the P1 pack Note Do not shake the excess P5P_L out of the vial. or - Using a volumetric pipette, withdraw 300 μL of P5P_L from the vial and carefully dispense it into well 1 of the ASTPLc P1 pack. 3. Carefully close well 1 of the ASTPLc P1 pack 4. To ensure homogeneity, mix the contents by gently swirling the P1 pack. Note Avoid formation of foam. Note Do not shake. 5. Repeat steps 1-4 for well 2 of the P1 pack with a fresh P5P_L vial. The ASTPLc R2 reagent is liquid and ready to use. Note: Select the X at the top of the page to return back to the course. Blank When preparing reagents, I always follow the directions in the IFU. The P5P reagent needed for the R1 Reagent is supplied ready to use in vials, it just needs to be transferred into the reagent pack and gently mixed. So, I just pour the contents of one vial into well 1 of the pack, then mix by gently swirling. I then repeat the same steps for well 2. I pour the second vial into well 2 and gently mix. Once both wells are mixed, the reagents can be loaded on the system. Troponin Troponin Calibrator Let me show an example of: Preparing a calibrator Blank Let me show an example of a calibrator that we recently had to prepare in our lab. I think this is one piece of information you are not going to want to miss. Troponin Calibrators Low Calibrator Ready to Use High Calibrator Needs preparation Note: Select the X at the top of the page to return back to the course. Blank Let’s look at the calibrators for Troponin. I am not going to go step by step thru the directions for this I just want to explain why these calibrators require some special handling. Special handling is required because the low-level calibrator for Troponin is liquid and ready to use, while the other calibrator, the high level is lyophilized and needs to be reconstituted. So again, this is a good example for my new hires to pay attention to the handling instructions in the IFU as it can affect the outcome of the calibrations and results. Question Bank 1 HOOD05162003349419 | 15 MAY 2023 2 Vitamin B12 2.1 Blank 2.2 Blank 3 Folate 3.1 Blank 3.2 Blank 4 P5P Method 4.1 Blank 4.2 Blank 5 Troponin 5.1 Blank 5.2 Blank