Dimension® Integrated Chemistry Systems LOCI® Online Training

Continue Continue Continue Continue Continue Continue Continue Continue Continue Continue Continue Untitled Scene Master Template HILS2218 | Effective Date: 25-Mar-2022 ? Dimension® Integrated Chemistry Systems LOCI® Online Training Learn about LOCI features, functionality, technology, and components across Dimension® EXL™ 200 Integrated Chemistry System and Dimension® EXL™ with LM Integrated Chemistry System. Identify the method used by LOCI® technology 1 Describe homogenous processing using LOCI® technology 4 Identify major LOCI® components 3 Identify key reactants 2 Welcome script: Welcome to Dimension Integrated Chemistry Systems LOCI Online Training. In this course, you will learn about the features and functions of LOCI technology across Dimension® EXL™ 200 Integrated Chemistry System and Dimension® EXL™ with LM Integrated Chemistry System. ? LOCI® Features The Luminescent Oxygen Channeling Immunoassay (LOCI®) technology measures specialty analytes such as TNIH, TSHL, and FT4L with increased sensitivity and accuracy. The immunoassay sandwich is prepared and briefly incubated on the HM incubation wheel and measurement takes place in the LOCI® read chamber. Homogeneous immunoassays do not require separation of immune complexes from unbound reactants. After a brief incubation, measurements take place in the LOCI® read chamber. LOCI Features image LOCIReader640x480.jpg script: LOCI® technology measures specialty analytes such as TNIH, TSHL, and free T4 with increased sensitivity and accuracy. The homogeneous immunoassay is prepared and briefly incubated on the incubation wheel, before being transferred to the LOCI® read chamber for measurement ? LOCI® Technology LOCI® chemiluminescence represents an important advance in immunochemistry because it pairs a highly sensitive immunoassay with a patented, state-of-the-art detection method. Select the button below to learn more about the use of LOCI® technology to detect immune complexes. Go LOCI Technology image LOCI_05_01_intro(1).jpg script: LOCI® chemiluminescence represents an important advance in immunochemistry because it pairs a highly sensitive immunoassay with a patented, state-of-the-art detection method. Select the “go” button to learn more about the use of LOCI technology to detect immune complexes. ? LOCI® Components Vessel Holder Vessel Track R2 Reagent Probe Incubate Wheel Photometric Sample Probe Reaction Vessel LOCI® Arm LOCI® Reader LED Light Source Select the numbered markers on the right to learn more about each component. LOCI® Reader The LOCI® arm transfers the vessel from incubate wheel to the LOCI® reader where the sample is analyzed. LOCI® Arm The LOCI® arm transfers the vessel from incubate wheel to the LOCI® reader. Photometric Sample Probe The photometric sample probe aspirates specimen from the sample area and delivers it to a reaction vessel. Reaction Vessel The photometric sample probe aspirates specimen from the sample area and delivers it to a reaction vessel. R2 Reagent Probe The R2 reagent probe aspirates reagents from a Flex® cartridge and delivers to a reaction vessel. Incubation Wheel The incubate wheel incubates reaction vessel contents to allow reactants to form an immunosandwich. The incubate wheel has 45 slots and maintains a temperature in the range of 37.3-39.6° C. Incubation times vary according to the particular LOCI® test being run. Vessel Track The vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel by the vessel transfer shuttle. Vessel Holder The vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel by the vessel transfer shuttle. LED Light Source Following a flash of light at 680nm, singlet oxygen is produced by the sensibeads. Singlet oxygen is diffused into bound chemibeads, producing chemiluminescence. Light produced is measured at 612nm. Signal produced is proportional to the amount of analyte present. LOCI Components base layer: Script: In this section, you will learn how the various LOCI® components function to process a sample. Select the numbered markers on the right to learn more about each component. image: EXL200_HMR2Area_640x480.jpg marker 1 image: VesselFeeder2_640.jpg marker 2 image: VesselFeeder2_640.jpg marker 3 image: R2DelHMWheel640.jpg marker 4 image: IncubateWheel640.jpg marker 5 image: SamplerToVessel640.jpg marker 6 image: SamplerToVessel640.jpg marker 7 image: LOCIArmToReader2_640(1).jpg marker 8 image: LOCIreader640.jpg marker 9 image: HMandLOCIWindow640.jpg ? Homogenous Processing using LOCI® Technology Follow the journey of a sample through homogenous processing using LOCI® technology by selecting the numbered steps below. hmandlociwindow640 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 Homogenous Processing using LOCI Technology base layer image: hmandlociwindow640.jpg script: Follow the journey of a sample through homogenous processing using LOCI® technology by selecting the numbered steps below. layer 1: image: vesselfeeder2.jpg script: First, the vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel. layer 2 image: incubatewheel640.jpg script: The R2 reagent probe aspirates and delivers reagents to the reaction vessel. layer 3 image: samplertovessel640.jpg script: The photometric sample probe aspirates and delivers sample to the same reaction vessel. layer 4 image: incubatewheel640.jpg script: The incubate wheel incubates reaction vessel content to allow reactants to form an immunosandwich. The incubate wheel has 45 slots and maintains a temperature of 37° C. Incubation times vary according to the particular LOCI® test being run. layer 5 image: lociarmtoreader2.jpg script: The LOCI® arm transfers the reaction vessel from the incubate wheel to the LOCI® reader. layer 6 image: exlhmlcloci_640.jpg script: Finally, following a flash of light at 680nm, singlet oxygen is produced by the sensibeads. Singlet oxygen is diffused into bound chemibeads, producing chemiluminescence. Light produced is measured at 612nm. The signal produced is proportional to the amount of analyte present. Finally, following a flash of light at 680nm, singlet oxygen is produced by the sensibeads. Singlet oxygen is diffused into bound chemibeads, producing chemiluminescence. Light produced is measured at 612nm. The signal produced is proportional to the amount of analyte present. 6 exlhmlcloci_640.jpg Homogenous Processing using LOCI® Technology The LOCI® arm transfers the reaction vessel from the incubate wheel to the LOCI® reader. 5 lociarmtoreader2.jpg Homogenous Processing using LOCI® Technology The incubate wheel incubates reaction vessel content to allow reactants to form an immunosandwich. The incubate wheel has 45 slots and maintains a temperature of 37° C. Incubation times vary according to the particular LOCI® test being run. 4 incubatewheel640 Homogenous Processing using LOCI® Technology The photometric sample probe aspirates and delivers sample to the same reaction vessel. 3 Homogenous Processing using LOCI® Technology Homogenous Processing using LOCI® Technology The R2 reagent probe aspirates and delivers reagents to the reaction vessel. 2 Homogenous Processing using LOCI® Technology First, the vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel. 1 vesselfeeder2640 ? Consumables Flex® reagent cartridges contain reagents necessary to perform LOCI® (left) and HM (right) testing, as well as others. Note color and lid cover of Flex® reagent cartridges which are used to protect reagents from light. EXL_FlexTNIandMMB_640x480.jpg Consumables script: Flex® reagent cartridges contain reagents necessary to perform LOCI® and HM testing, as well as others. Note that the color and lid cover of Flex® reagent cartridges are used to protect reagents from light. HM testing, features, and functionality are covered in a separate course. image: EXL_FlexTNIandMMB_640x480.jpg ? Course Review Congratulations. You have completed Dimension® Integrated Chemistry Systems LOCI® Online Training course. Select the numbered buttons below to review the material before proceeding to the final assessment. Identify major LOCI® components Describe homogenous processing using LOCI® technology 4 4 4 3 3 3 Identify key reactants Identify the method used by LOCI® technology 1 1 2 2 2 Course Review Describe Homogenous Processing Using LOCI® Technology First, the vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel. The R2 reagent probe aspirates and delivers reagents to the reaction vessel. The photometric sample probe aspirates and delivers sample to the same reaction vessel. The incubation wheel incubates reaction vessel content to allow reactants to form an immunosandwich. The LOCI® arm transfers the reaction vessel from the incubate wheel to the LOCI® reader. Lastly, following a flash of light at 680nm, singlet oxygen is produced by the sensibeads, then diffused into bound chemibeads, producing chemiluminescence. Note: Select the X in the top right corner to return to the course Identify major LOCI® Components LOCI® Reader The LOCI® arm transfers the vessel from incubate wheel to the LOCI® reader where the sample is analyzed. LOCI® Arm The LOCI® arm transfers the vessel from incubate wheel to the LOCI® reader. Photometric Sample Probe The photometric sample probe aspirates specimen from the sample area and delivers it to the same reaction vessel. Reaction Vessel The photometric sample probe aspirates specimen from the sample area and delivers it to the same reaction vessel. R2 Reagent Probe The R2 reagent probe aspirates reagents from a Flex® cartridge and delivers to a reaction vessel. Incubation Wheel The incubate wheel incubates reaction vessel contents to allow reactants to form an immunosandwich. The incubate wheel has 45 slots and maintains a temperature in the range of 37.3-39.6° C. Incubation times vary according to the particular LOCI® test being run. Vessel Track The vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel by the vessel transfer shuttle. Vessel Holder The vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel by the vessel transfer shuttle. LED Light Source Following a flash of light at 680nm, singlet oxygen is produced by the sensibeads. Singlet oxygen is diffused into bound chemibeads, producing chemiluminescence. Light produced is measured at 612nm. Signal produced is proportional to the amount of analyte present. Vessel Holder Vessel Track R2 Reagent Probe Incubate Wheel Photometric Sample Probe Reaction Vessel LOCI® Arm LOCI® Reader LED Light Source Note: Select the X in the top right corner to return to the course Identify Key Reactants Key Chemibead Monoclonal antibody Antigen (analyte in sample) Biotinylated antibody Biotin Streptavidin Sensibead loci_chemibeads.jpg Note: Select the X in the top right corner to return to the course When illuminated at a 680nm wavelength, Sensibeads generate singlet oxygen, which diffuses into nearby bound Chemibeads and triggers a chemiluminescent reaction. The resulting signal is measured at 612nm and is proportional to the concentration of analyte being tested. Identify the Method Used By LOCI® Technology exlhmlcloci_640.jpg Note: Select the X in the top right corner to return to the course Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. © Siemens Healthcare GmbH 2023 Siemens Healthineers Headquarters\Siemens Healthcare GmbH\Henkestr. 127\ 91052 Erlangen, Germany\Telephone: +49 9131 84-0\siemens-healthineers.com ? Disclaimer Assessment Start ? This assessment will test your retention of the presented content. A passing score of 80% or higher is required to complete the course and earn your certificate . Assessment questions must be answered completely to receive full credit. Partial credit will not be given for assessment questions that require multiple answers. You may repeat the assessment as many times as needed. Assessment Select the best answer. ? Chemiluminescence Turbidimetry Question 1 of 4 LOCI® technology involves which detection method? Bioluminescence Potentiometry Multiple Choice Incorrect Turbidimetry® is not measured by LOCI technology. Incorrect Incorrect Potentiometry is not measured by LOCI ® technology. Incorrect Bioluminescence is not measured by LOCI® technology. Select the best answer. ? Sensibead Chemibead Question 2 of 4 Which reactant is labeled by the number 7? Monoclonal Antibody Biotin loci_chemibeads.jpg Multiple Choice image: loci_chemibeads.jpg Incorrect Chemibead is labeled by the number 1. Incorrect Incorrect Biotin is labeled by the number 5. Incorrect Monoclonal Antibody is labeled by the number 2. Select the best answer. ? Photometric Sample Probe Vessel Holder Question 3 of 4 The hardware component labeled number 5 is the ________________. LOCI® Arm Vessel Track . Multiple Choice image: dimensionfam_maint_loci_hardware.jpg Incorrect The vessel holder is labeled number 1. Incorrect Incorrect The vessel track is labeled number 2. Incorrect The LOCI® arm is labeled number 7. The vessel holder feeds reaction vessels to the track system, where they are loaded onto the incubate wheel. The R2 reagent probe aspirates and delivers reagents to the reaction vessel. The photometric sample probe aspirates and delivers sample to the same reaction vessel. The incubate wheel incubates reaction vessel content to allow reactants to form an immunosandwich. What is the correct order of homogenous processing steps? Drag each response to place the list in the correct order. ? Question 4 of 4 Sequence Incorrect One or more answers are incorrect. Correct Assessment Results YOUR SCORE: PASSING SCORE: Review Retry Retry Continue Continue Continue %Results.ScorePercent%% %Results.PassPercent%% ? Assessment Results You did not pass the course. Take time to review the assessment then select Retry to continue. Congratulations. You passed the course.. Exit To access your Certificate of Completion, select the Launch button drop down on the course overview page. You can also access the certificate from your PEPconnect transcript. ? You have completed the Dimension® Integrated Chemistry Systems LOCI® Online Training Completion Navigation Help Select the icon above to open the table of contents. Click Next to continue. Next Welcome Slide The timeline displays the slide progression. Slide the orange bar backwards to rewind the timeline. Click Next to continue. Next Tmeline Select the CC icon to display closed captioning (subtitles). Click Next to continue. Next Caption Icon Select the buttons to learn more about a topic. Be sure to review all topics before navigating to the next slide. Click Next to continue. Next Tab Arrow Slide Select the X to close the pop-up. Click Next to continue. Next Layer Slide Some images may have a magnifier icon. Select the image to see an enlarged view. Select it again to return to the normal view. Click Next to continue. Next Zoom Slide Some images have a magnifier icon in the bottom-left corner. Select these image to see an enlarged view of the image. Select the image again to return to the normal view. Select Submit to record your response. Click the X in the upper right corner to exit the navigation help. Assessment Slide LOCI Technology ? LOCI® Technology LOCI® chemiluminescence represents an important advance in immunochemistry because it pairs a highly sensitive immunoassay with a patented, state-of-the-art detection method. LOCI® chemiluminescence involves the reactants shown above. Chemibeads Chemibeads Key Chemibead Monoclonal antibody Antigen (analyte in sample) Biotinylated antibody Biotin Streptavidin Sensibead Biotinylated Antibodies Biotinylated Antibodies Sensibeads+Streptavidin Sensibeads+Streptavidin Generating Chemiluminescence Generating Chemiluminescence Key LOCI® Reactants Key LOCI® Reactants Key LOCI® Reactants loci_chemibeads.jpg Select the X in the top right corner to return to the course Key LOCI Reactants image: loci_chemibeads.jpg script: LOCI® chemiluminescence involves the reactants shown on this slide. chemibeads layer: image: loci_chemibeads.jpg script: Non-competitive, or sandwich, immunoassays use method-specific synthetic Chemibeads that are coated with monoclonal antibodies specific for the analyte being tested. biotinylated antibodies layer: video: (web object) script: Immunoassay reactants include the analyte being tested, which serves as the antigen (Ag), as well as a second monoclonal antibody (Ab) specific for the analyte being tested. Biotin is attached to this antibody-it’s a biotinylated antibody. During incubation at 37° C, Ab + Ag + Ab immune complexes form, where antigen is “sandwiched” between monoclonal antibodies. sensibeads+streptavidin layer: video: web object script: Immunoassay reactants also include Sensibeads coated with streptavidin. Sensibeads contain a photosensitive dye. Streptavidin is a small protein with unique biochemical properties, such as its high affinity to bind to biotin. When added to the assay, streptavidin quickly binds to the biotin portion of the biotinylated antibody to form bead-pair immune complexes. Generating chemiluminescence layer: video: web object script: When illuminated at a 680nm wavelength, Sensibeads generate singlet oxygen, which diffuses into nearby bound Chemibeads and triggers a chemiluminescent reaction. The resulting signal is measured at 612nm and is proportional to the concentration of analyte being tested. When illuminated at a 680nm wavelength, Sensibeads generate singlet oxygen, which diffuses into nearby bound Chemibeads and triggers a chemiluminescent reaction. The resulting signal is measured at 612nm and is proportional to the concentration of analyte being tested. Select the X in the top right corner to return to the course Key LED light source Singlet oxygen released from Sensibead Chemiluminescence generated from Chemibead Generating Chemiluminescence Immunoassay reactants also include Sensibeads coated with streptavidin. Sensibeads contain a photosensitive dye. Streptavidin is a small protein with unique biochemical properties, such as its high affinity to bind to biotin. When added to the assay, streptavidin quickly binds to the biotin portion of the biotinylated antibody to form bead-pair immune complexes. Sensibeads+Streptavidin Key Chemibead Monoclonal antibody Antigen (analyte in sample) Biotinylated antibody Biotin Streptavidin Sensibead Select the X in the top right corner to return to the course Immunoassay reactants include the analyte being tested, which serves as the antigen (Ag), as well as a second monoclonal antibody (Ab) specific for the analyte being tested. Biotin is attached to this antibody-it’s a biotinylated antibody. During incubation at 37° C, Ab + Ag + Ab immune complexes form, where antigen is “sandwiched” between monoclonal antibodies. Key Chemibead Monoclonal antibody Antigen (analyte in sample) Biotinylated antibody Biotin Biotinylated Antibodies Select the X in the top right corner to return to the course Non-competitive (sandwich) immunoassays use method-specific synthetic Chemibeads that are coated with monoclonal antibodies specific for the analyte being tested. Chemibeads Select the X in the top right corner to return to the course Key Chemibead Monoclonal antibody loci_chemibeads.jpg Question Bank 1 dimensionsystems_systemoverview_loci_olt 1.1 Welcome 1.2 LOCI Features 1.3 LOCI Technology 1.4 LOCI Components 1.5 Homogenous Processing using LOCI Technology 1.6 Consumables 1.7 Course Review 1.8 Disclaimer 1.9 Assessment