HM Reactions and Features on the Dimension® Family of Integrated Chemistry Systems Video

Welcome to the HM reactions and features on the dimension family of Integrated Chemistry Systems video. In this video you will learn how immunoassays are measured on the dimension systems within the heterogeneous immunoassay module HM. Describe the HM reaction and processing steps and identify the HM key components and functions. The heterogeneous immunoassay module processes chromium based immunoassays for the measurement of specialty analytes of low molecular weight such as human chorionic gonadotropin HCG. The immunoassay is prepared in the HMM area where it is washed, mixed and incubated. The photometric sample probe delivers the immunoassay reactants into cuvets where the test results are read photometrically. Other HMM module features include disposable reaction vessels to set up the immunoassay, automatic loading of reaction vessels into the incubate wheel via the vessel transfer arm and track system. Chromium based antibody with high sensitivity increased processing throughput by performing immunoassay steps concurrently rather than sequentially. All flex reagent cartridges used for HM testing have two common components, chromium dioxide coated with capture antibody and conjugate tag antibody reagent as reagent components. These antibodies are used to detect or measure specific antigens in a sample. Antigens are the analytes that will be measured during the heterogeneous immunoassay. The type of immunoassay formats used for the HMM methods depends on the particular analyte being tested. Refer to the Flex reagent Cartridge Instructions for use IFU for details about each HMM test method. In this example, let's process an HMM test request for a mass creatine kinase, MB isoenzyme or MMB level. To start the assay, there are two reagent probads. Chromium dioxide from the Flex reagent cartridge to the reaction vessel on the incubate wheel. Chromium dioxide is coated with capture antibody specific for the analyte being tested. Next, the photometric sample probe adds patient or QC sample from the sample wheel into the same reaction vessel on the incubate wheel. The reaction vessel now contains capture antibody plus sample. The R2 reagent probe adds conjugate reagent from the Flex reagent cartridge to the reaction vessel. The conjugate tag antibody is specific for the analyte being tested. The reaction vessel now contains capture antibody sample and tag antibody. The vessel reactants incubate on the incubate wheel at 37°. During this time an immuno sandwich containing capture antibody sample tag antibody forms. Incubation times will vary according to the particular HM test being run. After incubation, the reaction vessel is transferred to the wash wheel for magnetic separation. Because chromium dioxide is magnetic, the 12 stationary magnets positioned around the wash wheel pull only the chromium dioxide containing immuno sandwich plus excess chromium dioxide aside, Wash probe 1 uses the longer aspirate probe to remove sample and unbound conjugate excess MMB tag antibody. The only conjugate antibody remaining is that which is bound to the MMB analyte in the sample and is part of the immunosandwich. The amount of bound conjugate antibody is directly proportional to the amount of MMB in the sample. Not all chromium dioxide antibody binds to sample MMB. Remember that excess chromium dioxide antibody still remains in the reaction vessel. To wash the bound reactants and facilitate removing unbound conjugate antibody, Wash Probe 1 uses the shorter probe to add chemistry wash to the reaction vessel. The two vessel mixers on the wash wheel resuspend the bound and unbound reactants. The reaction vessel now has completed one wash cycle for each immunoassay. The HM module will complete a total of four wash cycles to remove all excess conjugate. Wash probe one will complete two wash cycles and then wash probe 2 will complete the remaining two wash cycles. The R1 reagent probe delivers reagent from the flex reagent cartridge into cuvets to prepare for the photometric Reed. The photometric sample probe transfers the washed immuno sandwiches from the reaction vessels to cuvets in the cuvette. A colorometric reaction takes place and test results are read photometrically. The color produced is proportional to the amount of analyte in the sample.

HM Reactions and Features on the Dimension® Family of Integrated Chemistry Systems Video Heterogeneous Immunoassay Module (HM) Features · Processes chromium-based immunoassays for the measurement of specialty analytes of low molecular weight . The immunoassay is prepared in the HM area, and the final results are read photometrically after transferring reactants from reaction vessels to cuvettes HM Consumables All Flex® reagent cartridges used for HM testing have 2 components in common: · Chromium dioxide, or CRO2, coated with capture antibody · Conjugate/tag antibody reagent MMB SIEMENS Processing HM Methods The type of immunoassay formats used for the HM methods depends on the particular analyte being tested. Tag Ab CrO2Capture CPRG CRPG Diluent CrO2 Diluent Ab Flex® reagent cartridge Note: Refer to the Flex® reagent cartridge Instructions for Use (IFU) sheet for details about each HM test method. R2 reagent probe adds chromium-based antibody to the reaction vessel on the incubate wheel CrO2 added by R2 from Flex®® reagent cartridge Photometric sample probe adds sample to the same reaction vessel Sample containing analyte added from cup or tube R2 reagent probe adds conjugate/tag antibody to the reaction vessel Conjugate added by R2 reagent probe from Flex®® Vessel reactants incubate at 37℃ to form immuno-sandwiches (captured and tagged MMB) Excess CrO2 Excess Conjugate Wash wheel magnets are used to separate the reactants Wash Probe #1 uses the longer probe to aspirate unbound sample and conjugate Wash Probe #1 uses the shorter probe to add chemistry wash to the reaction vessel The 2 vessel mixers on the wash wheel re-suspend the bound and unbound reactants Wash Cycle Steps Step 1: In the wash wheel area, stationary magnets separate bound and unbound reactants in the reaction vessel Step 2: Wash probe aspirates unbound Wash Probe #2 reactants Step 3: Wash probe dispenses chemistry wash reagent to wash the bound reactants and remaining unbound reactants Step 4: Vessel mixers re-suspend the bound and unbound reactants in the reaction vessel Step 5: HM module repeats this process until 4 wash cycles have been completed The R1 reagent probe delivers reagent from the Flex® reagent cartridge into cuvettes to prepare for the photometric read C-reactive Protein Gradient, CPRG, added from Flex® reagent cartridge CRPG The photometric sample probe transfers the washed immuno-sandwiches from the reaction vessels to cuvettes Immuno-sandwich and excess CrO2 added In the cuvette, a colorimetric reaction takes place and test results are read photometrically. The color produced is proportional to the amount of analyte in the sample. CPRG (non-absorbing CPR color (absorbs at 577nm) CPR Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively "Operator Manual") issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively "Functionality") may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. Unrestricted | Published by Siemens Healthineers AG | @ Siemens Healthineers AG, 2024 Siemens Healthineers HQ | Siemens Healthineers AG Siemensstr. 3 91301 Forchheim Germany Phone: +49 9191 18-0 siemens-healthineers.com