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Hematek® 3000 Troubleshooting Job Aid

This job aid explains common troubleshooting procedures for the Hematek® 3000 slide stainer.

Hematek 3000 Troubleshooting Job Aid A. Troubleshooting Chart Problem Probable Cause Corrective Measure Main switch is not set to position I Set the main switch to position I Power cord is not plugged into the Plug the power cord into the socket socket at the rear of the system at the rear of the system System does not Power cord is not plugged into a Plug the power cord into a power start when the power outlet outlet with a ground contact power switch is Line fuses are blown Replace the line fuses pressed Circuit breaker in electrical circuit of building is tripped Reset the circuit breaker Electrical failure of the system Contact Siemens Healthineers Service Yellow low stain light goes on Hematek Stain Pak is empty Install a new Hematek Stain Pak Yellow low stain Hematek Stain Pak is not properly Reposition the Hematek Stain Pak in light goes on, positioned in the pocket at the the pocket so it moves freely up and but Hematek rear of the system down on the right end Stain Pak is not Fill level detector is not operating Contact Siemens Healthineers empty correctly service Empty, rinse and replace the waste Red waste tank Waste tank is full tank light goes on Waste tank is removed Empty, rinse and replace the waste tank Pump tubing is unlocked Set the pump tubing release knob to the lock position Hematek Stain Pak is empty Install a new Hematek Stain Pak Sensing switch fingers are not Check the adjustment of the sensing properly adjusted switch fingers Cannula openings are clogged Remove cannulas from the Hematek Stain Pak and clean away debris Pumps run but Cannulas are damaged Replace cannulas do not deliver Pump tubing has been pulled off Connect the tubes to the cannulas or fluids the cannulas or the nipples the nipples Pump tubing is clogged, worn, or perforated Replace the pump tubing Underplaten tubing has been pulled off the spouts under the Connect the tubes to the spouts platen under the platen Underplaten tubing is clogged, worn, or perforated Replace the underplaten tubing One or more pumps do not Sensing switch fingers are not Check the adjustment of the sensing properly adjusted switch fingers run Sensing switch is defective Contact Siemens Healthineers Service Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 SIEMENS Master Template Effective Date 10 Aug 2021 | HILS 2224 Job Aid Effective Date: 14 Oct 2022 | HOOD05162003307412 Healthineers Page 1 of 6 Job Aid | Hematek 3000 Maintenance Effective Date: 14 Oct 2022 | HOOD05162003307412 Problem Probable Cause Corrective Measure Fluids overflow on either side of Pump volumes are not correct Correct the pump volumes a glass slide System was accidentally unplugged, or power failure Remove all glass slides from the occurred during staining platen and reprocess them System is not properly leveled Level the system Mixing grooves on the platen are dirty Clean the platen Staining is Platen has not been primed Load priming slides before staining uneven before staining glass slides smeared glass slides Fluids are not evenly delivered Vent the bottles of the Hematek Stain Pak Stain tube or cannula is clogged Clean the stain tube and cannula Pump tubing is worn or perforated Replace the pump tubing Pump volumes are not correct Correct the pump volumes System was accidentally unplugged, or power failure Remove all glass slides from the occurred during staining platen and reprocess them Pump tubing has not been primed before staining smeared glass Prime the pump tubing slides Hematek Stain Pak is deteriorated or outdated Install a new Hematek Stain Pak Sensing switch fingers are not Check the adjustment of the sensing Staining is pale properly adjusted switch fingers Proper stain-to-buffer ratio has not been achieved Check the stain-to-buffer ratio Rinse volume is too high Check the rinse volume together with the stain-to-buffer ratio Stain volume is adjusted too high. Increasing stain volume may decrease stain intensity because Correct the pump volumes of an incorrect stain-to-buffer ratio Glass slides stick together Glass slide drying system does not Contact Siemens Healthineers because they operate properly Service are not dry Conveyor spirals do not operate N/A Contact Siemens Healthineers Service SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 2 of 6 Job Aid | Hematek 3000 Maintenance Effective Date: 14 Oct 2022 | HOOD05162003307412 B. How to solve common problems When stained blood smears are not of the desired quality, the first tendency is to assume the fluids or the slide stainer is at fault. However, other factors could be the cause of the poor quality of stained blood smears. The following list can help isolate the problem prior to checking the system: • Was the blood thoroughly mixed before making the smear? • Is the blood old or does it contain an incorrect anticoagulant? • Is the blood smear too thick, too thin, or does it spread unevenly? • Is the feathered edge of the smear end at least 1.5cm from the end of the glass slide? • Was the blood smear thoroughly dry before staining? • Are the glass slides clean? Even new glass slides are not necessarily clean. • Are the glass slides flat? Variable thickness of a given glass slide can cause uneven staining. • Is the microscope clean and adequately illuminated? A dirty microscope or inadequate lighting can give the impression of a poorly stained blood smear. Checking the adjustment of the sensing switch fingers Load 4 spare glass slides into the grooves of the conveyor spirals. Watch the system perform the staining. Has the stain pump activated after approximately 50% of a glass slide width has passed over the stain orifice? YES NO Has the buffer pump activated after about 3mm (1/8in) of a glass slide has crossed over the buffer orifice? NO YES Has the rinse pump activated just as the leading edge of a glass slide NO crosses over the rinse orifice? YES Adjust the sensing switch fingers You can now load smeared glass slides Refer to Instruction Manual for details SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 3 of 6 Job Aid | Hematek 3000 Maintenance Effective Date: 14 Oct 2022 | HOOD05162003307412 Problem: Poor staining or incorrect pump volumes When glass slides are not satisfactorily stained or pump volumes do not appear to be optimum, check the staining process first. If necessary, correct and verify the pump volumes. Check the staining process When glass slides are not satisfactorily stained or pump volumes do not appear to be optimum, check the staining process first. If necessary, correct and verify the pump volumes. Note: After completing each of the following steps, load several spare smeared glass slides and check the result. If any of these steps corrects the situation, you do not need to correct the pump volumes. 1. Raise the lid. 2. At the right side of the system, observe the circular bubble level to check if the leveling feet have been properly adjusted and the system is level. The bubble in the circular bubble level should be centered within the inscribed circle. If not, level the system using the two leveling knobs located under the instrument at the right and left corners. Refer to Instruction Manual for details. 3. Make sure the Hematek Stain Pak has been vented. 4. Clean the platen. 5. Make sure that there is no residue in the two mixing grooves. 6. Clean the drain troughs. 7. Check the adjustment of the sensing switch fingers. 8. Replace the pump tubing. 9. Replace the underplaten tubing. 10. If none of these checks solve the problem, correct the pump volumes. Leveling Bubble SIEMENS Leveling knobs SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 4 of 6 Job Aid | Hematek 3000 Maintenance Effective Date: 14 Oct 2022 | HOOD05162003307412 Correcting the pump volumes The slide stainer has 3 pump assemblies, one for each fluid. An adjustment knob that controls the rotating speed of the respective pump is located next to each pump assembly. The volume of fluid delivered by each pump can be adjusted by turning the respective adjustment knob clockwise to increase the volume or counter-clockwise to decrease the volume. Each adjustment knob features a centering catch which provides a central reference point. Pump Assemblies and Adjustment Knobs Pump Cap 3 2) Pump Arm (2 +2 3 Pump Arm Handle 1 4 . +2 Adjustment Knob 4 +6 Volume Increments 1. Volume increments are marked on each adjustment knob. 2. The stain-to-buffer ratio is adjustable to provide ratios of 1:2 to 1:3. 3. This feature allows the user flexibility in obtaining the desired stain results. A small change in the stain-to-buffer ratio can result in either lighter or darker staining. Adjustment Knob Value of Volume Increment Stain 0.01 mL Buffer 0.01 mL Rinse 0.05 mL SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 5 of 6 Job Aid | Hematek 3000 Maintenance Effective Date: 14 Oct 2022 | HOOD05162003307412 Please note this learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. © Siemens Healthcare GmbH 2022 Siemens Healthineers Headquarters Siemens Healthcare GmbH Henkestr. 127 91052 Erlangen, Germany Phone: +49 9131 84-0 siemens-healthineers.com SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 6 of 6

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