Hematek® 3000 System Overview Job Aid

Hematek 3000 System Overview Job Aid Description The Hematek slide stainer is a fully automated, bench-top hematology system for in vitro diagnostic use in the clinical laboratory. It automatically stains prepared blood or bone marrow smears on standard glass microscope slides. The system accepts, conveys, fixes, stains, and delivers dry blood or bone marrow smear preparations that are spread on standard thickness 25mm x 75mm glass slides. The glass slides are stained at the rate of one glass slide per minute. SIEMENS Hematek 2IEWEN2 HOWUSI6K Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 SIEMENS Master Template Effective Date 10 Aug 2021 | HILS 2224 Job Aid Effective Date: 14 Oct 2022 | HOOD05162003307403 Healthineers Page 1 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Principle of Operation As the glass slides move along the platen, with the smeared side down, the system performs the following steps: 1. Two conveyor spirals move the slides from right to left down the length of the platen. 2. Raised edges called rails hold the slides at a precise distance. This spacing forms a capillary space into which stain, buffer and rinse are delivered. 3. As the glass slide moves down the platen it contacts the first sensing switch finger which then activates the stain pump to start the delivery of stain. 4. When the glass slide reaches the second sensing switch finger the buffer pump is activated. The stain and buffer move around the mixing grooves and hence fully intermingle. 5. When the glass slide contacts the third sensing switch finger the rinse pump starts delivering the rinse. The rinse washes off the stain and buffer mixture and cleans the slide of precipitate. 6. As the slide moves, the capillary space under the slide attracts the solution thus keeping the slide in contact with the solution. 7. The relevant pumps deliver a specific amount of stain, buffer, and rinse, according to the setting of their adjustment knobs. The surplus of fluids is drained into the waste tank. 8. The final section is just rails. The slide is supported while it is dried by a flow of air from a low velocity blower. The slide should be dry when it falls into the slide drawer. The system produces stained glass slides of consistent quality in a continuous process. This is accomplished by having a fixed length of time in each of the three phases of stain, buffer, and rinse, as well as a predetermined ratio of stain-to-buffer volumes in the buffer phase. A properly stained glass slide is the result of an interactive process involving the pump volumes, the mixing process, and the stain-to-buffer ratio. The system is pre-set to a stain-to- buffer ratio of approximately 1:2.5 and a rinse volume of approximately 1.0mL per glass slide when each adjustment knob is at its centering catch. The stain-to-buffer ratio can be adjusted from 1:2 to 1:3. Hematek SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 2 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Identification of instrument components When looking at the Instruction Manual most of the figures show the rear of the 33 instrument becasuse it makes most of the components more visible. Hinges 1 Stain adjustment knob 2 Stain pump assembly Power plug 3 Buffer adjustment knob 4 Buffer pump assembly 5 Rinse adjustment knob 6 Rinse pump assembly 7 Priming button 8 Pump tubing release knob The On/OFF switch is in the middle of the instrument on the right hand side. Once the instrument is on, the spiral conveyors should begin to rotate immediately. Note – look for the green illuminated light to ensure the instrument is on. On/Off Switch SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 3 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Pump Assemblies Each pump assembly consists of a pump motor and four rollers that are attached to the underside of a pump cap. A pump arm that presses the stain, buffer or rinse tube against the pump is situated next to each pump assembly. The volume of fluid delivered by each pump can be adjusted by turning the respective adjustment knob clockwise to increase the volume or counter-clockwise to decrease the volume. The rinse pump, buffer pump, Rinse Pump Connection and stain pump will be rotating Stain Pump when the instrument is on. Buffer Pump Connection Connection Adjust knob on the left hand side that will engage the arms to cause friction on the peristaltic pump to begin moving liquid. The knobs to the right of each pump are used to adjust ratios. Reagent Priming The reagent priming button is located on the left hand side of the instrument. This will start all three pumps priming to get all of the air out of the pumps between the runs. Do not stop priming before the pump tubing is free of air bubbles. SIEMOGS Reagent priming Button SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 4 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Pump Tube Set The Hematek Pump Tube Set consists of three tubes, each labeled with an identifying number specific to the fluid carried by the tube. Each tube has a clear plastic cuff that fits snugly up to the pump arm and holds the tube in position to prevent slippage. Each tube is a specific diameter and length to ensure precise measurement of the fluids. Push the pump until the clear cuff gets to the pump. Stain Low and Waste Tank Lights The yellow Low Stain light and the red Waste Tank Full light are in the middle of the instrument on the right hand side. SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 5 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Workflow 1. Two conveyor spirals move the slide on a platen. 2. Three sensing switches activate when the slide moves. 3. Each switch activates a solution pump. 4. Stain, buffer, or rinse are delivered into the capillary space between the slide and the platen. 5. After staining and rinsing the slide is air dried by a low velocity air blower. 6. The slide is then deposited in the slide drawer. 2mbGLELOZE LOZE SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 6 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Workflow - Load Slide 1. Mark four glass slides as priming slides (e.g., Label them with the word “prime” using a permanent marker). 2. Process the priming slides every time before you start staining smeared glass slides. After staining, wipe the priming slide clean and store for re-use whenever you prime the instrument. The slide loading lines are inscribed on the right side of the platen. They indicate the correct placing of the glass slides in the conveyor spirals. The frosted/blood side is placed onto the platen facing the left. Glass slides are moved by the conveyor spirals from right to left, first at the vertical plane, then at the horizontal plane with the blood smear facing downward toward the platen. Note: If glass slides are not placed correctly in the grooves of the conveyor spirals, this can cause them to break. Damage to the system can result. Stain Port Buffer Port anbei Lot Rinse O SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 7 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Stain Pak Each stain pack should stain 750 to 1000 slides depending upon the user defined stain-to- buffer ratio. Hematek® Stain Pak Modified Wright's Stain Colorant de Wright modifié --- --- - Modifizierte Wright-Färbung Colorazione di Wright modificata Hematek Stain Pak Colorante de Wright modificado Corante modificado de Wright Wright-Giemsa Stain Colorant Wright-Giemsa Andret Wright-farve Wright-Giemsa-Färbung Modifierad Wright-färgning Colorazione di Wright-Giemsa Hematek (125-151590 REF 06609653 Colorante de Wright-Giemsa SIEMENS SMN 10:19/1965 Corante de Wright-Giemsa Wright-Giemsa-farve Wright-Giemsa-färgning Hematek 54h-143 -25420095 SIEMENS LA 1001 4/58 CE tt Hematek Cannula Set The Hematek Cannula Set contains three cannulas. The cannulas are inserted into the Hematek Stain Pak bottles and connected to the stain, buffer, or rinse tubes. When installing a Stain Pak, punch a hole and remove the cannula. Rotate the cannula 90 degrees and repunch the Pack for venting purposes. Refer to the Hematek Stain Pak labeling and to Material Safety Data Sheets for details concerning any special precautions related to the disposal of Hematek Stain Paks. Material Safety Data Sheets are available from the Siemens website. SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Healthineers Master Template Effective Date 10 Aug 2021 | HILS 2224 Page 8 of 9 Job Aid | Hematek 3000 System Overview Effective Date: 14 Oct 2022 | HOOD05162003307403 Please note this learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. © Siemens Healthcare GmbH 2022 Siemens Healthineers Headquarters Siemens Healthcare GmbH Henkestr. 127 91052 Erlangen, Germany Phone: +49 9131 84-0 siemens-healthineers.com SIEMENS Unrestricted | Published by Siemens Healthcare GmbH © Siemens Healthcare GmbH, 2022 Master Template Effective Date 10 Aug 2021 | HILS 2224 Healthineers Page 9 of 9