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Dimension® RxL® Max Integrated Chemistry System IMT Overview Online Training

Learn about the Integrated Multisensor Technology (IMT) components and functions, IMT fluidics, and electrolyte measurement.

Continue Continue Continue Untitled Scene Master Template HILS2218| Effective Date: 25 Mar 2022 ? Dimension® RxL Max® Integrated Chemistry System IMT Overview Online Training Learn about the Integrated Multisensor Technology (IMT) components and functions, IMT fluidics and electrolyte measurement Identify components of the IMT system and their functions Trace the flow of samples from the 1:10 dilution through the IMT system 1 2 Describe how electrolytes are measured in the QuikLYTE® Integrated Multisensor 3 Welcome Welcome to the Dimension® RxL Max® Integrated Chemistry System IMT Overview Online Training. In this course you will learn about the Integrated Multisensor Technology, or IMT components and functions on the Dimension® RxL Max® Integrated Chemistry System. You will also learn about IMT fluidics and electrolyte measurement. This course will cover the following three learning objectives. dimensionfam_rxlmax_imtoverview_olt_1.mp3 ? IMT Features, Components and Functions IMT system features include: QuikLYTE® Integrated Multisensor which measures Na, K, and CI Small sample volume requiring only 40 µL of serum, plasma or urine Indirect sample measurement using a 1:10 dilution No daily maintenance Rapid turnaround time for results: 57.6 seconds Select the numbered steps below to learn more about IMT components and their functions. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 8 8 8 9 9 9 IMT Features Components and Functions Base Layer: In this section you will learn about IMT features, components and functions. The IMT system features the QuikLYTE® Integrated Multisensor, which measures the electrolytes sodium, potassium and chloride.   A 40 microliter sample of serum, plasma or urine is prepared as a 1:10 dilution for indirect measurement. Results are available in 57.6 seconds and no daily maintenance is required. Select the numbered steps below to learn more about IMT components and their functions. dimensionfam_rxlmax_imtoverview_olt_2a.mp3 IMT Pump: The IMT Pump moves samples and fluids through the IMT system. Select the numbered steps to continue. dimensionfam_rxlmax_imtoverview_olt_2b.mp3 QuikLYTE® Integrated Multisensor: The IMT tower holds the QuikLYTE® Integrated Multisensor, which includes two main components within the cartridge housing: the ceramic chip with sodium, potassium, chloride and reference electrodes, and the elastomer flow channel for the sample and Salt Bridge Solution to pass through separately. Select the markers on the image to identify the QuikLYTE® Integrated Multisensor components. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2c.mp3 IMT Sample Probe & IMT Port: The IMT Sample Probe aspirates and delivers samples to the IMT port, level senses samples in tubes, and detects fluid in Sample Cups.The IMT Port is the receptacle where the 1:10 sample dilution is mixed. Select the markers on the image to identify the IMT components. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2d.mp3 IMT Rotary Valve: The IMT Rotary Valve controls air and fluid segments that enter the IMT system. Select the marker on the image to identify the IMT component. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2e.mp3 IMT Diluent Pump: The IMT Diluent Pump controls the amount of sample diluent delivered to the IMT port for the 1:10 sample dilution. Select the marker on the image to identify the IMT Diluent Pump. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2f.mp3 QuikLYTE Sample Diluent and Salt Bridge Solution: QuikLYTE® Sample Diluent is used to make the 1:10 sample dilution. Salt Bridge Solution is a reference solution that provides a constant electrical potential for electrolyte measurements. Select the markers on the image to identify the QuikLYTE® Sample Diluent and Salt Bridge Solution. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2g.mp3 IMT Fluids: Standards A and B (prepared as 1:10 dilutions) are used for IMT system calibrations. Standard A is also used for the pump alignment, and a 1 point calibration drift check after each sample. No additional dilutions of the standards are made by the IMT system. Flush solution is used to clean and rinse the IMT port and to separate samples in the IMT tubing to prevent carryover. Select the markers on the image to identify the IMT fluids. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2h.mp3 Flush Pump: The flush pump delivers flush solution to the IMT port. Select the marker on the image to identify the flush pump. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_2i.mp3 IMT Monopump: The IMT Monopump controls the fluids aspirated for the 1:10 dilution. Three tubings are attached to the Monopump and connect to the IMT Sample Probe, System Water bottle and the IMT Drain. Select the marker on the image to identify the IMT Monopump. Then select the X in the upper right corner to continue. dimensionfam_rxlmax_imtoverview_olt_2j.mp3 IMT Monopump IMT Monopump Controls the fluids aspirated for the 1:10 dilution. Three tubings are attached to the Monopump and connected to the: IMT Sample Probe System Water bottle IMT Drain 9 Flush Pump Flush Pump Delivers flush solution to the IMT port 8 IMT Fluids 7 Flush Solution Used to clean and rinse the IMT port and to separate samples in the IMT tubing to prevent carryover Standards A and B (prepared as 1:10 dilutions) are used for IMT system calibrations. Standard A is also used for the pump alignment, and a 1 point calibration drift check after each sample. No additional dilutions of the standards are made by the IMT system. QuikLYTE® Sample Diluent & Salt Bridge Solution 6 QuikLYTE® Sample Diluent Used to make the 1:10 sample dilution Salt Bridge Solution Reference solution that provides a constant electrical potential for electrolyte measurements IMT Diluent Pump 5 IMT Diluent Pump Controls the amount of sample diluent delivered to the IMT port for the 1:10 sample dilution IMT Rotary Valve 4 IMT Rotary Valve Controls air and fluid segments that enter the IMT system IMT Sample Probe & IMT Port 3 IMT Sample Probe Aspirates and delivers samples to the IMT port Level senses samples in tubes Detects fluid in Sample Cups IMT Port Receptacle where the 1:10 sample dilution is mixed QuikLYTE® Integrated Multisensor 2 Ceramic Chip (QuikLYTE® Integrated Multisensor) IMT Tower IMT Pump 1 Knowledge Check You have just learned about IMT features, components and functions. Select Start to test your knowledge of the presented content. Start ? Knowledge Check Select the applicable image. ? Which component contains the QuikLYTE® Integrated Multisensor? Question 1 of 3 Pick One Correct Incorrect Incorrect Select the applicable image. ? Which solution(s) are used for IMT system calibrations? Question 2 of 3 Pick One Correct Incorrect Incorrect Select the applicable image. ? Which component delivers flush solution to the IMT port? Question 3 of 3 Pick One Correct Incorrect Incorrect Knowledge Check Completion Review Retry Retry Continue Continue ? You have just completed the Knowledge Check. Select Review to assess how your responses compare to the correct answers. Select Retry to test your knowledge again or select Continue to advance through the course. Knowledge Check Completion ? IMT Fluidics IMT components are connected by a series of tubings that carry fluid and air segments through the system. Understanding how the components are connected and what happens in each tubing section will help you to: Monitor the IMT system during electrolyte processing Perform IMT maintenance Troubleshoot IMT error messages Select the tab arrows to learn more about IMT Fluidics. IMT Flow Diagram & Components IMT Sampling & Fluid Flow IMT 1:10 Sample Dilution Process IMT Fluid Flow IMT Fluidics Fluid flows through the IMT system via a series of tubings and connections. Understanding the fluidics will help you monitor electrolyte processing, perform maintenance, and troubleshoot IMT error messages. Select the tab arrows to learn more about IMT Fluidics. dimensionfam_rxlmax_imtoverview_olt_8.mp3 IMT Fluid Flow Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments. Liquids pulled into the system include the following: 1:10 diluted sample for measurement Standards A or B for calibrations Flush solution to clean the port and tubings to prevent carryover from one sample to the next The process begins as the IMT pump pulls liquid into the IMT rotary valve, which rotates to insert a segment of air from the air inlet port into the liquid stream, followed by flush solution from the IMT port. This action creates a pattern of air, sample, and flush liquid segments that move through the tubing for measurement by the QuikLYTE® Integrated Multisensor. Note: The IMT system creates the same air/liquid pattern for all liquids that flow through the system: serum, plasma, or urine, as well as Standards A and B. Flush Air Air Diluted Sample or Standard Air Air Reading takes place here Correct Air/Fluid Placement Air ? IMT Sampling and Fluid Flow IMT sampling begins with the 1:10 dilution of patient or QC samples in the IMT port. The IMT pump pulls the samples through the system in an alternating pattern of liquid and air segments Key features of the fluid flow process include: The IMT system performs an indirect measurement. All samples are diluted 1:10 prior to being measured Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments The IMT system pulls air and liquids through the system using the peristaltic action of the IMT pump Flush Air Air Diluted Sample or Standard Air Air Air Reading takes place here Correct Air/Fluid Placement ? ? IMT Measurements In this section, you will learn how electrolytes are measured in the QuikLYTE® Integrated Multisensor after samples are moved into the tower. Select the numbered steps below to learn more about IMT measurements. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 IMT Measurements Base Layer: In this section, you will learn how electrolytes are measured in the QuikLYTE® Integrated Multisensor after samples are moved into the tower. Select the numbered steps below to learn more about IMT measurements. dimensionfam_rxlmax_imtoverview_olt_9a.mp3 Sample Flow: Let's follow a sample as it enters the IMT system to learn how electrolytes are measured. First, the IMT pump draws the diluted sample from the IMT port through X0 tubing to the IMT rotary valve. From there, the diluted sample flows through the X1 tubing into the IMT tower. Select the numbered markers on the image to follow the sample flow. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_9b.mp3 Positioning Sample: In the IMT tower, the sample portion of the liquid segment is positioned across the QuikLYTE® Integrated Multisensor. The QuikLYTE® Integrated Multisensor takes continuous conductivity readings to verify sample positioning. The IMT pump performs an automatic pump alignment to ensure proper positioning of the sample. Select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_9c.mp3 IMT Pump: The movement of the air and liquid through the system is timed by the IMT pump so that the sensors take readings exactly when the liquid segment, which could be sample or standard or air segment is positioned in the QuikLYTE® Integrated Multisensor. A different part of the sensor (reference electrode) does the same for the Salt Bridge Solution. Select the numbered markers on the image. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_9d.mp3 Multisensor Readings: The QuikLYTE® Integrated Multisensor readings determine when the previous sample has been removed by detecting that air is present, and when new sample is present by detecting that a liquid segment is present. The potential of each sodium, potassium and chloride ion selective electrode is measured against the reference electrode to determine electrolyte levels in the sample. Salt Bridge Solution, which flows through R1 tubing serves as the reference solution. Select the numbered markers on the image to identify the QuikLYTE® Integrated Multisensor components. Then select the numbered steps below to continue. dimensionfam_rxlmax_imtoverview_olt_9e.mp3 Exiting the IMT System: After measurement, the Salt Bridge Solution and diluted sample exit the IMT tower through the X2 tubing and around the IMT pump through the X tubing to the waste tubing. Select the numbered markers on the image to identify the X2 and waste tubing. Then select the X in the upper right corner to continue. dimensionfam_rxlmax_imtoverview_olt_9f.mp3 Exiting the IMT System After measurement, the Salt Bridge Solution and diluted sample exit the IMT tower through the X2 tubing (1) and around the IMT pump through X tubing to the waste tubing (2). Select the numbered markers on the image to identify the X2 and waste tubing. Then select the X in the upper right corner to continue. 5 X2 Tubing Waste Tubing QuikLYTE® Integrated Multisensor Readings QuikLYTE® Integrated Multisensor readings determine when: The previous sample has been removed by detecting that air is present A new sample is present by detecting that a liquid segment is present The potential of each Na, K, and Cl ion selective electrode is measured against the reference electrode to determine electrolyte levels in the sample. Select the numbered markers on the image to identify the QuikLYTE® Integrated Multisensor components. Then select the numbered steps below to continue. 4 Air/Liquid Sensor Reference Electrode IMT Pump The movement of the air and liquid through the system is controlled by the IMT pump. Sensors take readings exactly when the liquid segment (sample or standard) or air segment is positioned in the QuikLYTE® Integrated Multisensor. Select the numbered markers on the image. Then select the numbered steps below to continue. 3 IMT Pump QuikLYTE® Integrated Multisensor Positioning Sample In the IMT tower, the sample portion of the liquid segment is positioned across the QuikLYTE® Integrated Multisensor. The Multisensor takes continuous conductivity readings to verify sample positioning. Select the numbered steps below to continue. 2 Sample Flow The IMT pump draws the diluted sample from the IMT port (1) through X0 tubing (2) to the IMT rotary valve. From there, the diluted sample flows through the X1(3) tubing into the IMT tower. Select the numbered markers on the image to follow the sample flow. Then select steps below to continue. 1 IMT Port X0 Tubing X1 Tubing ? Course Review Congratulations. You have completed the Dimension® RxL Max® Integrated Chemistry System IMT Overview Online Training. Select the numbered buttons below to review the material before proceeding to the final assessment. Describe how electrolytes are measured in the QuikLYTE® Integrated Multisensor Trace the flow of samples from the 1:10 dilution through the IMT system Identify components of the IMT system and their functions 1 1 2 2 2 3 3 3 Course Review Describe how electrolytes are measured in the QuikLYTE® Integrated Multisensor Electrolytes are measured in the QuikLYTE® Integrated Multisensor after samples are moved into the IMT tower using the following process: Sample Flow-the IMT pump draws the diluted sample from the IMT port through the X0 tubing to the IMT rotary valve. From there, the diluted sample flows through the X1 tubing into the IMT tower. Positioning Sample-in the IMT tower, the sample portion of the liquid segment is positioned across the QuikLYTE® Integrated Multisensor, which takes continuous conductivity readings to verify sample positioning. IMT Pump-the movement of the air and liquid through the system is controlled by the IMT pump. Sensors take readings exactly when the liquid segment (sample or standard) is positioned in the QuikLYTE® Integrated Multisensor. QuikLYTE® Integrated Multisensor Readings-determine when the previous sample has been removed by detecting that air is present and when new sample is present by detecting that a liquid segment is present. The potential of each sodium, potassium and chloride ion selective electrode is measured against the reference electrode to determine electrolyte levels in the sample. The Salt Bridge Solution, which flows through the R1 tubing serves as the reference solution. Exiting the IMT System-after measurement, the Salt Bridge Solution and diluted sample exit the IMT tower through the X2 tubing and around the IMT pump through the X tubing to the waste tubing. Select the X in the upper-right corner to continue. ? Trace the flow of samples from the 1:10 dilution through the IMT system The following key features of the fluid flow process are: The IMT system performs an indirect measurement. All samples are diluted 1:10 prior to being measured. The IMT diluent pump delivers QuikLYTE® Sample Diluent to the IMT port for rinsing and then aspiration by the IMT Sample Probe. The IMT Sample Probe aspirates 290 µL of diluent then aspirates 40 µL of sample and aspirates 70 µL of diluent. The IMT Port is drained and flushed with Flush Solution. The IMT Sample Probe rapidly dispenses the 1:10 dilution into the IMT Port. The IMT pump pulls the diluted sample into the IMT system. Air and liquid detectors check for proper sample placement in the QuikLYTE® Integrated Multisensor. Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments. Liquids pulled into the system include the following: 1:10 diluted sample for measurement Standards A or B for calibrations Flush Solution to clean tubing and prevent carryover from one sample to the next The process begins as the IMT pump pulls liquid into the IMT rotary valve, which rotates to insert a segment of air from the air inlet port into the liquid stream, followed by flush solution from the IMT port. This action creates a pattern of air, sample, and flush liquid segments that move through the tubing for measurement by the QuikLYTE® Integrated Multisensor. Note: The IMT system creates the same air/liquid pattern for all liquids that flow through the system: serum, plasma, or urine, as well as Standards A and B. The IMT system pulls air and liquids through the system using the peristaltic action of the IMT pump. Select the X in the upper-right corner to continue. ? Identify components of the IMT system and their functions IMT system features include: QuikLYTE® Integrated Multisensor which measures Na, K, and CI Small sample volume requiring only 40 µL of serum, plasma or urine Indirect sample measurement using a 1:10 dilution No daily maintenance IMT Components and Functions include: The IMT pump moves samples and fluids through the IMT system. The IMT tower holds the QuikLYTE® Integrated Multisensor. The two main components within the cartridge housing are: Ceramic sensor substrate chip with Na, K, Cl, and reference electrodes Elastomer flow channel for the standard or patient sample and Salt Bridge Solution to pass through separately The IMT Sample Probe aspirates and delivers samples to the IMT port, level senses samples in tubes and detects fluid in sample cups. The IMT port is the receptacle where the 1:10 sample dilution is mixed. The IMT rotary valve controls air and fluid segments that enter the IMT system. The IMT diluent pump controls the amount of sample diluent delivered to the IMT port for the 1:10 sample dilution. The QuikLYTE® Sample Diluent is used to make the 1:10 sample dilution. The Salt Bridge Solution is a reference solution that provides a constant electrical potential for electrolyte measurements. The IMT fluids Standards A and B are used for IMT system calibrations. No additional dilutions of the standards are made by the IMT system. Flush Solution is used to clean and rinse the IMT port and to separate samples to prevent carryover. The flush pump delivers Flush Solution to the IMT port. The IMT Monopump-controls the fluids aspirated for the 1:10 dilution. Select the X in the upper-right corner to continue. ? Disclaimer Disclaimer Assessment Start ? This assessment will test your retention of the presented content. A passing score of 80% or higher is required to complete the course and earn your certificate . Assessment questions must be answered completely to receive full credit. Partial credit will not be given for assessment questions that require multiple answers. You may repeat the assessment as many times as needed. Assessment Select three (3) answers. ? Question 1 of 7 Which of the following is true regarding IMT sampling and fluid flow? The IMT system performs an indirect measurement. All samples are diluted 1:10 prior to being measured Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments The rotary valve dilutes and mixes the samples before a measurement takes place The IMT system uses the peristaltic action of the IMT pump to pull air and liquids through the system Multiple Answer Incorrect One or more answers are incorrect. Correct Select the best answer. ? Question 2 of 7 How does air enter the IMT system? Through the rotary valve Through the IMT port Through the X2 tubing Through the QuikLYTE® Integrated Multisensor Multiple Choice Incorrect Incorrect Incorrect Incorrect Select the best answer. ? Question 3 of 7 What does the QuikLYTE® Integrated Multisensor measure? The potential of each sodium, potassium and chloride electrode against the reference electrode to determine electrolyte in the sample The accuracy and precision of the 1:10 sample dilution The positioning of sample across the QuikLYTE® Integrated Multisensor Multiple Choice Incorrect Incorrect Incorrect Select the best answer. ? Question 4 of 7 Where does the sample and flush solution go after it exits the IMT port? IMT rotary valve IMT pump QuikLYTE® Integrated Multisensor Diluent pump Multiple Choice Incorrect Incorrect Incorrect Incorrect Select the best answer. ? Question 5 of 7 Which component controls the movement of air and liquid through the system? IMT pump Rotary valve IMT port Flush pump Multiple Choice Incorrect Incorrect Incorrect Incorrect Select the best answer. ? Question 6 of 7 Where is the 1:10 sample dilution mixed? IMT Port Photometric Sample Probe IMT Diluent Pump IMT Rotary Valve Multiple Choice Incorrect Incorrect Incorrect Incorrect Select the best answer. ? Question 7 of 7 Which component directs the flow of each fluid as it enter the IMT system? IMT Rotary Valve IMT Pump Flush Pump IMT Port Multiple Choice Incorrect Incorrect Incorrect Incorrect Assessment Results YOUR SCORE: PASSING SCORE: Review Retry Retry Continue Continue Continue %Results.ScorePercent%% %Results.PassPercent%% ? Assessment Results You did not pass the course. Take time to review the assessment then select Retry to continue. Congratulations. You passed the course.. Exit To access your Certificate of Completion, select the Certificates tab from the learning activity overview page. You can also access the certificate from your PEPconnect transcript. ? You have completed the Dimension® RxL Max® Integrated Chemistry System IMT Overview Online Training. Completion Navigation Help Select the icon above to open the table of contents. Click Next to continue. Next Welcome Slide The timeline displays the slide progression. Slide the orange bar backwards to rewind the timeline. Click Next to continue. Next Timeline Select the CC icon to display closed captioning (subtitles). Click Next to continue. Next Caption Icon add subtitles Select the buttons to learn more about a topic. Be sure to review all topics before navigating to the next slide. Click Next to continue. Next Tab Arrow Slide Select the X to close the pop-up. Click Next to continue. Next Layer Slide Select Submit to record your response. Click the X in the upper right corner to exit the navigation help. Assessment Slide Fluidics Chart ? IMT Flow Diagram and Component ID Chart This diagram is located in the Appendix of the Operator’s Guide and inside the instrument left cabinet door. Hover over the colored callouts on the diagram to learn more about IMT flow and components. Then select the X in the upper right corner to continue. IMT Flow Diagram Hover over the colored callouts on the diagram to learn more about IMT flow and components. dimensionfam_rxlmax_imtoverview_olt_8a1.mp3 IMT Diluent Pump At the IMT diluent pump, diluent enters the pump through D1 tubing (green) and exits through D2 tubing (green) to the IMT port IMT Rotary Valve The IMT Rotary Valve controls the entry of air and liquids into the IMT system: Liquids and air enter the rotary valve through the A tubing (Standard A), B tubing (Standard B) and X0 tubing (diluted sample and Flush) and air inlet port Air and liquid segments exit the rotary valve through the X1 tubing (black) QuikLYTE® Integrated Multisensor IMT Tower which houses the QuikLYTE® Integrated Multisensor: Patient and standard samples enter the sample channel through the X1 tubing (black) Salt Bridge Solution enters the reference channel through the R1 tubing (blue) Both samples and Salt Bridge Solution exit the sensor through the X2 tubing (black) Flush Solution Flush solution connects to the Flush pump through the F1 tubing and enters the IMT port through the F2 tubing IMT Pump X tubing stretches around the IMT pump from the X2 tubing to the X3 tubing Flush Pump The flush pump connects to flush solution through the F1 tubing (yellow), and connects to the IMT port through the F2 tubing (yellow) Standard A Standard A is delivered to the IMT rotary valve through the A tubing Standard B Standard B is delivered to the IMT rotary valve through the B tubing Salt Bridge Solution Salt Bridge Solution is pumped to the reference channel of the QuikLYTE® Integrated Multisensor through the R1 tubing (blue). The R1 tubing is seated inside the pinch valve on the back of the IMT tower QuikLYTE® Sample Diluent Sample Diluent connects to the diluent pump through the D1 tubing (green) and enters the IMT port through the D2 tubing (green) IMT Port In the IMT Port: Diluent enters through D2 tubing (green) Flush enters through F2 tubing (yellow) Sample exits through X0 tubing (red) 1:10 Sample Dilu Process ? IMT 1:10 Sample Dilution Process The IMT system performs an indirect measurement on all samples, which means all patient and control samples are diluted 1:10 prior to being measured. Select the numbered steps below to learn more about the 1:10 sample dilution process. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 8 8 8 IMT 1:10 Sample Dilution Process IMT Sampling begins with the 1:10 dilution of patient or QC samples in the IMT Port. The IMT Pump pulls the samples through the system in an alternating pattern of liquid and air segments. Select the numbered steps below to learn more about the 1:10 sample dilution process. dimensionfam_rxlmax_imtoverview_olt_8b.mp3 Circuit Checks for Liquid Replacement Air and liquid detectors check for proper sample placement in the QuikLYTE® Integrated Multisensor. Select the X in the upper right corner to continue. 8 Sample Enters IMT System The IMT Pump pulls the diluted sample into the IMT system. Select the numbered steps below to continue. 7 IMT Sample Probe Dispenses 1:10 Dilution The IMT Sample Probe rapidly dispenses the 1:10 dilution into the IMT Port. The conical shape of the port aids in the mixing process. Select the numbered steps below to continue. 6 IMT Port is Drained and Flushed The IMT Port is flushed with Flush Solution. Select the numbered steps below to continue. 5 IMT Sample Probe Aspirates Diluent The IMT Sample probe aspirates 70 µL of Sample Diluent. Select the numbered steps below to continue. 4 IMT Sample Probe Aspirates Sample The IMT Sample probe aspirates 40 µL of sample. Select the numbered steps below to continue. 3 IMT Sample Probe Aspirates Diluent The IMT Sample Probe aspirates 290 µL of Sample Diluent. Select the numbered steps below to continue. 2 Diluent Delivered to IMT Port The IMT diluent pump delivers QuikLYTE® Sample Diluent to the IMT Port for rinsing and then for aspiration by the IMT Sample Probe. Select the marker on the image to identify the IMT Port. Then select the numbered steps below to continue. 1 IMT Port Question Bank 1 QR700000806 | Effective Date: 20 Sep 2023 1.1 Welcome 1.2 IMT Features Components and Functions 1.3 Knowledge Check 1.8 IMT Fluidics 1.9 IMT Measurements 1.10 Course Review 1.12 Assessment 1.21 Completion

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