Atellica® Solution Immunoassay Principles Online Training

Continue Continue Continue Continue Course Content Master Template HILS2218 | Effective Date: 25-Mar-2022 ? Atellica® Solution Immunoassay Principles Online Training In this online training, we will look in depth at the core immunoassay technologies and assay reaction formats on the Atellica® Solution. Identify the assay formats 1 Describe the principles of chemiluminescent technology 2 Welcome 1.1_welcome.mp3 Welcome to the Atellica Solution Immunoassay Principles Online Training course. In this online training, we will look in depth at the core immunoassay technology and assay reaction formats in use on the Atellica Solution. This course will cover the following two learning objectives. For navigation help, select the question mark button located in the lower right corner of each slide. In the next few slides, we will talk about the chemiluminescent technology, in which we will define and identify the functions of Paramagnetic Particles (PMP), Acridinium Ester (AE), as well as the three different assay formats, Sandwich, Competitive, and Antibody-Capture. ? Chemiluminescent Technology What is Chemiluminescense? Chemical reaction that emits energy in the form of light The light the reaction produces indicates the amount of analyte in a sample Select the tab arrows to learn more about some of the key components involved in the Chemiluminescent Technology and their integral role in Immunoassay testing. Primary Reagent Process Chemiluminescent Technology 1.2_baselayer.mp3 The core immunoassay technology in use on the Atellica Solution is based on the principle of Chemiluminescence. Chemiluminescence describes a chemical reaction that emits energy in the form of light. The light the reaction produces is measured in Relative Light Units, or RLUs, has a proportional relationship to the amount of analyte in a sample. 1.2-primaryreagent.mp3 Select the button below to learn more about Acridinium Ester. 1.2-process.mp3 The immunoassay Atellica Solution are based on the formation of an immune complex. To measure the target analyte in the sample, specifically formulated reagents are added in predetermined steps of a test sequence. Acridinium Ester (AE) Siemens Healthineers Acridinium Ester (AE) is an evolving chemiluminescent technology, using customized molecules for advanced assay design. Table with 1 columns and 2 rows Technology Benefits Small molecular size Increased stability Longer reagent shelf life Increased Precision Increased Sensitivity Smaller sample volume Process During incubation, coated PMPs, AE, and the target antigen or antibody bind together During washing, the cuvette is exposed to a magnetic field and the PMPs are drawn towards the magnets, while the unbound sample and reagent are washed away Acid containing hydrogen peroxide is added to the cuvette, followed by base reagent (to create the basic environment necessary for the chemiluminescent reaction to occur) The sudden change of environment allows the hydrogen peroxide to rapidly oxidize the AE and produce a flash of light PMP-Ab complex AE-labeled Ag AE-labeled Ab Analyte-specific Ag in sample PMP-Ab-Ag-Ab-AE complex Primary Reagent The primary reagents in use contain 2 essential parts: ​ Note: Latex Core Magnetic Particles are also used Learn more Learn more Learn more Table with 1 columns and 2 rows Paramagnetic Particles (PMP) Iron oxide crystals Solid Phase of Primary Reagent Coated with antibody or antigen Table with 1 columns and 2 rows Acridinium Ester (AE) Produces light Light reagent of primary reagent Bound to antibody or antigen ? Assay Formats Three assay reaction formats take advantage of the selective biochemical characteristics of antibodies binding to their specific corresponding antigens. Direct → the concentration of an analyte increases as RLU’s increase Indirect → the concentration of analyte decreases as RLU’s increase Select the tab arrows to learn more about the steps of each assay format. Sandwich Format Competitive Format Antibody-Capture Format Assay Formats 1.3_assayformats.mp3 The three assay reaction formats, Sandwich, Competitive, and Antibody Capture, take advantage of the selective biochemical characteristic of antibodies binding to their specific corresponding antigens. Because Paramagnetic Particles and Acridinium Ester are contained in the immune complex formed with the target analyte, the light emitted during the assay reaction is proportional, either directly or indirectly, to the concentration of the target in the sample. Directly proportional assays, Sandwich, and Antibody Capture Formats, result in the concentration of an analyte increasing as the light reading increases. Conversely, an indirectly proportional assay, Competitive format, results in the concentration of an analyte decreasing as the light reading increases. Antibody-Capture Format Competitive Format Sandwich Format ? Course Review Congratulations. You have completed the Atellica® Solution Immunoassay Principles Online Training course. Select the numbered buttons below to review the material before proceeding to the final assessment. Describe the principles of chemiluminescent technology Identify the assay formats 1 1 2 2 2 Course Review Identify the assay formats Within the chemiluminescent technology found on the Atellica® Solution, there are three assay reaction formats: Sandwich Format - directly proportional Competitive Format - indirectly proportional Antibody-Capture Format - directly proportional Describe the principles of chemiluminescent technology Chemiluminescent Technology: Chemiluminescence = the chemical reaction that emits energy in the form of light which indicates amount of analyte in sample The Primary reagents in use contain two essential parts: Paramagnetic Particles (PMPs) Acridinium Ester (AE) Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. © Siemens Healthcare GmbH 2023 Siemens Healthineers Headquarters\Siemens Healthcare GmbH\Henkestr. 127\ 91052 Erlangen, Germany\Telephone: +49 9131 84-0\siemens-healthineers.com ? Disclaimer Assessment Start ? This assessment will test your retention of the presented content. A passing score of 80% or higher is required to complete the course and earn your certificate . Assessment questions must be answered completely to receive full credit. Partial credit will not be given for assessment questions that require multiple answers. You may repeat the assessment as many times as needed. Assessment Select the best answer. ? Question 1 of 5 What is the light reagent of the primary reagent used in the Chemiluminescent technology? Acridinium Ester Acridinium Ester Paramagnetic Particles NAD+ G6PDH Multiple Choice Incorrect Incorrect Incorrect Correct Select the best answer. ? Question 2 of 5 In which of the three chemiluminescent assay formats is the light emitted, indirectly proportional to the concentration of the target analyte in the sample? Competitive Competitive Sandwich Antibody-Capture Multiple Choice Incorrect Incorrect Correct Select the best answer. ? Question 3 of 5 In which chemiluminescent format is the lite reagent not dispensed into the reaction cuvette until after the cuvette is exposed to a magnetic field? Antibody-Capture Antibody-Capture Sandwich Competitive Multiple Choice Incorrect Incorrect Correct Select the best answer to complete the statement. ? Question 4 of 5 The light that a reaction produces is measured in ________. Relative Light Units Relative Light Units Reactive Light Units Real Light Units Reaction Light Output Multiple Choice Incorrect Incorrect Incorrect Correct Select the best answer. ? Question 5 of 5 Which of the three chemiluminescent assay formats is used to detect TSH, CEA, and hCG assays? Sandwich Format Sandwich Format Antibody-Capture Format Competitive Format Multiple Choice Incorrect Incorrect Correct Assessment Results YOUR SCORE: PASSING SCORE: Review Retry Retry Continue Continue Continue %Results.ScorePercent%% %Results.PassPercent%% ? Assessment Results You did not pass the course. Take time to review the assessment then select Retry to continue. Congratulations. You passed the course.. Exit To access your Certificate of Completion, select the Launch button drop down on the course overview page. You can also access the certificate from your PEPconnect transcript. ? You have completed the Atellica® Solution Immunoassay Principles Online Training. Completion Navigation Help Select the icon above to open the table of contents. Click Next to continue. Next Welcome Slide The timeline displays the slide progression. Slide the orange bar backwards to rewind the timeline. Click Next to continue. Next Timeline Select the CC icon to display closed captioning (subtitles). Click Next to continue. Next Caption Icon Select the buttons to learn more about a topic. Be sure to review all topics before navigating to the next slide. Click Next to continue. Next Tab Arrow Slide Select the X to close the pop-up. Click Next to continue. Next Layer Slide Select Submit to record your response. Click the X in the upper right corner to exit the navigation help. Assessment Slide Question Bank 1 HOOD05162003327479 | Effective Date: 26-JAN-2023 1.1 Welcome 1.2 Chemiluminescent Technology 1.3 Assay Formats 1.4 Course Review 1.5 Disclaimer 1.6 Assessment