Atellica® IM Analyzer Hardware Overview Online Training

Continue Continue Continue Continue Continue Continue Main Content Master Template HILS2218 | Effective Date: 25 Mar 2022 ? Atellica® IM Analyzer Hardware Overview Online Training The Atellica Solution consists of a family of modules and analyzers that are configurable for individual laboratory testing needs. This course will introduce you to the hardware components on the immunoassay analyzer. Identify hardware components and their functions 1 Describe the test processing sequence 2 Welcome Welcome to the Atellica IM Analyzer Hardware Overview Online Training course. The Atellica Solution is a family of modules and analyzers consisting of a central workstation/computer, sample entry, and analyzers that are configurable for the individual laboratory testing needs. In this online training you’ll learn about the hardware components on the immunoassay analyzer. NOTE: Please refer to the Atellica Solution Operator's Guide for information on safety precautions that should be followed when operating the system. This course will cover the following two learning objectives. For navigation help, select the question mark button located in the lower right corner of each slide. ? Hardware Components The external components can be accessed while the system is running. The interior components are accessed by opening the top cover, which can only be done when the system is not processing samples. Select the button below to learn more about these hardware components. System Components Hardware Components In this next section, the main areas of the Atellica IM Analyzer will be discussed. The external components can be accessed while the system is running. The interior components are accessed by opening the top cover, which can only be done when the system is not processing samples. Please select the button "System Components" on the Overview tab to learn more about these hardware components. ? Test Processing Sequence By following the journey that cuvettes take from beginning to end as a test is processed, you will gain a better perspective on how the different parts of the system function. Select the numbers below to learn more about each steps of the test processing sequence. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 8 8 8 Test Processing Sequence By following the journey that cuvettes take from beginning to end as a test is processed, you will gain a better perspective on how the different parts of the system function. Let’s open the top cover and look at each step of the test processing sequence for the Atellica IM Analyzer in detail. step1 The first step of the test processing sequence is the transfer of cuvettes from the cuvette bin to the incubation ring. The system takes the cuvettes from the cuvette loading bin via the conveyor. At the top of the conveyor, cuvettes are positioned upright in the orientation chute. Once a cuvette reaches the end of the orientation chute, it drops down the vertical chute into the cuvette channel assembly. Cuvettes are moved through the cuvette channel assembly by the cuvette pusher and enter the incubation ring at the ring loader position. The system internal air temperature is controlled to 33°C. The heaters used to control the internal air temperature also serve to pre-warm the cuvettes to 33°C prior to the cuvettes entering the incubation ring. step2 While in the incubator, the cuvettes are maintained at 37°C. Sample, ancillary and primary reagents are dispensed into the cuvette while the cuvette is in the incubation ring. When a test begins processing, the sample probe first picks up a tip from the sample tip tray. The sample probe then aspirates sample from the sample tube and transfers the sample into the cuvette in the incubation ring. After dispensing, the tip is removed from the sample probe. The tip falls down the tip waste chute into the solid waste bin. The reagent probes then dispense primary and ancillary reagent into the cuvette. step3 The primary reagent contains paramagnetic particles (PMPs) and Acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the Acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test. One of the reagents probes aspirates reagent from the reagent compartment and then the probe dispenses the reagent into the cuvette in the incubation ring. The cuvette incubates in the incubation ring for a time specified by the assay test. Longer incubation assays are transported to the inner incubation ring to complete their incubation, while shorter incubation assays stay in the outer incubation ring for the duration of their incubation. During incubation, the analyte being measured in the sample and the acridinium ester label are bound to the PMPs. step4 Following incubation, the cuvettes are elevated to the wash ring. The unbound sample and reagent are washed away from the PMPs in the cuvette in the wash ring. The wash fluid is heated to 34°C as it is dispensed into the cuvette through the dispense ports. Magnets positioned along the wash ring pull the PMPs to the side of the cuvette. Aspirate probes then draw fluid out of the cuvette leaving the PMPs with the bound material behind. The number of wash cycles is determined by the assay test definition. After the final wash, fluid is aspirated out of the cuvette, acid reagent is added and the cuvette is elevated into the luminometer. step5 The luminometer contains a photomultiplier tube (PMT) with photon-counting electronics, a base probe, and a waste probe. The base probe is located beneath the luminometer cover. When the cuvette enters the luminometer, base reagent is dispensed into the cuvette. The base reagent is heated to 34°C it is dispensed. The addition of the base to the cuvette changes the chemical environment to cause the chemiluminescent reaction to occur as the Acridinium ester oxidizes. step6 The PMT measures the light emitted as relative light units (RLUs). The system software converts the RLU raw signal into test results. step7 Following the reaction, the liquid waste is aspirated from the cuvette by the waste probe. step8 And the cuvette is discarded down the cuvette waste chute into the solid waste bin. 8 The cuvette falls into the solid waste bin. 7 Liquid waste is removed from the cuvette. 6 The photomultiplier tube (PMT) takes the chemiluminescent measurement. 5 Acid and base are dispensed into the cuvette. 4 The paramagnetic particles (PMPs) in the cuvette are washed in the wash ring. 3 The cuvette incubates in the incubation ring. 2 Primary and ancillary reagent are transferred into the cuvette. 1 Sample is transferred into the cuvette. ? Course Review Congratulations. You have completed the Atellica® IM Analyzer Hardware Overview Online Training course. Select the numbered buttons below to review the material before proceeding to the final assessment. Describe the test processing sequence Identify hardware components and their functions 1 1 2 2 Course Review Describe the test processing sequence The cuvette is moved to the luminometer and base is added. The base reagent is heated to 34°C as it is dispensed. The addition of the base to the cuvette changes the chemical environment to cause the chemiluminescent reaction to occur as the acridinium ester oxidizes The PMT takes the chemiluminescent measurement The PMT measures the light emitted as relative light units (RLUs) The system software converts the RLU raw signal into test results Liquid waste is removed from the cuvette The cuvette falls into the solid waste bin Describe the test processing sequence The cuvette incubates in the incubation ring for a time specified by the assay test. Longer incubation assays are transported to the inner incubation ring to complete their incubation, while shorter incubation assays stay in the outer incubation ring for the duration of their incubation. During incubation, the analyte being measured in the sample and the acridinium ester label are bound to the PMPs The PMPs in the cuvette are washed in the wash ring and acid is added Following incubation, the incubation ring elevates the cuvettes to the wash ring. The unbound sample and reagent are washed away from the PMPs in the cuvette in the wash ring. The wash fluid is heated to 34°C as it is dispensed into the cuvette through the dispense ports. Magnets positioned along the wash ring pull the PMPs to the side of the cuvette. Aspirate probes then draw fluid out of the cuvette leaving the PMPs with the bound material behind. The number of wash cycles is determined by the assay test definition After the final wash, fluid is aspirated out of the cuvette and acid is added Describe the test processing sequence The steps of the test processing sequence: Sample is transferred into the cuvette The sample probe first picks up a tip from the sample tip tray The sample probe then aspirates sample from the sample tube and transfers the sample into the cuvette in the incubation ring After dispensing, the tip is removed from the sample probe. The tip falls down the tip waste chute into the solid waste bin Primary and ancillary reagent are transferred into the cuvette. The primary reagent contains paramagnetic particles (PMPs) and Acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the Acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test One of the reagents probes aspirates reagents from the reagent compartment The probe then dispenses the reagent into the cuvette in the incubation ring Describe the test processing sequence Transfer of cuvettes from the cuvette bin to the incubation ring: The system takes the cuvettes from the cuvette loading bin via the conveyor At the top of the conveyor, cuvettes are positioned upright in the orientation chute Once a cuvette reaches the end of the orientation chute, it drops down the vertical chute into the cuvette channel assembly Cuvettes are moved through the cuvette channel assembly by the cuvette pusher and drop down into the incubation ring at the ring loader position. The system internal air temperature is controlled to 33°C. The heaters used to control the internal air temperature also serve to pre-warm the cuvettes to 33°C prior to the cuvettes entering the incubation ring. While in the incubator, the cuvettes are maintained at 37°C Identify hardware components and their functions Interior Components (continue) Reagent probes: three reagent probes are used to deliver primary and ancillary reagent to the cuvettes. The probes are washed after each dispense Incubation ring: a circular, insulated track comprised of 2 independently movable rings that advance the cuvettes to the sample and reagent positions. Temperature is maintained at 37°C Sample probe: transfers sample from sample tube to a cuvette in the incubation ring Identify hardware components and their functions Interior Components Sample tip loader: maximum capacity of 1800 sample tips Wash ring: a circular track that washes and prepares reaction cuvettes for the chemiluminescent reaction. Also location of the acid probe Luminometer: contains the photomultiplier tube (PMT) with photon-counting electronics, base probe, and waste probe. Location where the chemiluminescent reaction occurs Cuvette bin: maximum capacity of 1500 cuvettes Reagent drawer: ability to load up to five primary/ancillary reagent packs at a time Reagent compartment: stores both primary and ancillary reagent packs in a single refrigerated tray. The temperature of the reagent compartment is maintained at 4° to 8°C. The compartment can hold up to 42 primary reagent packs and 35 ancillary reagent packs Identify hardware components and their functions Exterior Components System front and back cover: controlled by interlocks. Operator can unlock using a button in the software Sample tip loading lid: used to load additional packs of sample tips Cuvette bin lid: used to load additional cuvettes Reagent drawer: used to load up to 5 reagent packs at a time, and to remove empty reagent packs Solid waste area: used to remove cuvette waste, sample tip waste, and tip tray waste System fluid drawer: used to replace the IM Acid, IM Base, IM Wash, and IM Cleaner bottles Water and liquid waste area: contains one reservoir for water and three reservoirs for waste Fan filter: there is one fan filter located in the center rear panel Main power switch: used to power off and on the analyzer Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. Atellica, and all associated marks are trademarks of Siemens Healthcare Diagnostics Inc. or its affiliates. All other trademarks and brands are the property of their respective owners. © Siemens Healthcare GmbH 2023 Siemens Healthineers Headquarters\Siemens Healthcare GmbH\Henkestr. 127\ 91052 Erlangen, Germany\Telephone: +49 9131 84-0\siemens-healthineers.com ? Disclaimer Assessment Start ? This assessment will test your retention of the presented content. A passing score of 80% or higher is required to complete the course and earn your certificate . Assessment questions must be answered completely to receive full credit. Partial credit will not be given for assessment questions that require multiple answers. You may repeat the assessment as many times as needed. Assessment Select the best answer. ? Sample Probe Question 1 of 5 Which probe on the Atellica IM Analyzer uses disposable tips? Reagent Probe Aspirate Probe Multiple Choice Incorrect Incorrect The aspirate probes do not use disposable tips. Incorrect The reagent probe is washes in between each dispense and does not use disposable tips. ? Select three (3) answers. Question 2 of 5 Which components are found within the luminometer on the Atellica IM Analyzer? Photomultiplier Tube Base Probe Acid Probe Waste Probe Multiple Answer Incorrect One or more answers are incorrect. Correct Select the best answer. ? 28 days 3 months 3 months 3 months Question 3 of 5 On the Atellica IM Analyzer, which is the onboard stability of the IM Acid, IM Base, and IM Wash system fluids? 7 days 14 days Multiple Choice Incorrect Incorrect The onboard stability of the IM Acid, IM Base, and IM Wash system fluids is less than 3 months. Incorrect The onboard stability of the IM Acid, IM Base, and IM Wash system fluids is longer than 14 days. Incorrect The onboard stability of the IM Acid, IM Base, and IM Wash system fluids is longer than 7 days. Select the best answer. ? The cuvette travels to the wash ring, where the unbound sample and reagent are washed away from the paramagnetic particles. The cuvette travels along the cuvette channel assembly, where it is heated to 37°C. Question 4 of 5 During the Atellica IM Analyzer test processing sequence, which is the next step after incubation is complete? The cuvette travels to the luminometer, where the chemiluminescent reaction is measured. The cuvette travels to the dispense port, where ancillary reagent is added to the cuvette. Multiple Choice Incorrect Incorrect The cuvette travels along the cuvette channel assembly prior to entering the incubation ring. Incorrect If needed, ancillary reagent is added before incubation. Incorrect Before the chemiluminescent reaction occurs, the cuvettes are washed, and acid and base are added. Select the best answer. ? When the operator selects a software button to unlock the covers Question 5 of 5 When can the top covers of the Atellica IM Analyzer be opened by the operator? Only when the analyzer is in a Diagnostics state to perform maintenance or troubleshooting procedures Only when the system is powered down Multiple Choice Incorrect Incorrect The analyzer does not need to be powered down to open the top covers. Incorrect The analyzer does not need to be in a Diagnostics state to open the top covers. Assessment Results YOUR SCORE: PASSING SCORE: Review Retry Retry Continue Continue Continue %Results.ScorePercent%% %Results.PassPercent%% ? Assessment Results You did not pass the course. Take time to review the assessment then select Retry to continue. Congratulations. You passed the course.. Exit To access your Certificate of Completion, select the Certificates tab from the learning activity overview page. You can also access the certificate from your PEPconnect transcript. ? You have completed the Atellica® IM Analyzer Hardware Overview Online Training. Completion Navigation Help Select the icon above to open the table of contents. Click Next to continue. Next Welcome Slide The timeline displays the slide progression. Slide the orange bar backwards to rewind the timeline. Click Next to continue. Next Tmeline Select the CC icon to display closed captioning (subtitles). Click Next to continue. Next Caption Icon Select the buttons to learn more about a topic. Be sure to review all topics before navigating to the next slide. Click Next to continue. Next Tab Arrow Slide Select the X to close the pop-up. Click Next to continue. Next Layer Slide Some images may have a magnifier icon. Select the image to see an enlarged view. Select it again to return to the normal view. Click Next to continue. Next Zoom Slide Some images have a magnifier icon in the bottom-left corner. Select these image to see an enlarged view of the image. Select the image again to return to the normal view. Videos should default to the optimal resolution. However, to change the video resolution select the gear icon. Click Next to continue. Next Video Slide Some images have a magnifier icon in the bottom-left corner. Select these image to see an enlarged view of the image. Select the image again to return to the normal view. Select Submit to record your response. Click the X in the upper right corner to exit the navigation help. Assessment Slide System Components Note: Please turn on the closed captions. System Components The Atellica IM Analyzer is an automated immunoassay analyzer. The top system cover must be closed for the system to process samples. There are several access doors that the operator can open to access supplies, even while the system is running. Let’s look at each of these access doors. The sample tip loading lid is located on the left side of the top system cover. Blank This lid is used to load additional packs of sample tips into the system. Blank The cuvette bin lid is located on the top of the top system cover. Blank This lid is used to load additional cuvettes into the system. Blank The reagent drawer is located on the right side of the top system cover. Blank This drawer is used to load additional reagent packs onto the system, and to remove empty reagent packs from the system. Blank The water and liquid waste drawer is located on the bottom right side of the system. Blank This drawer contains one reservoir for water and three reservoirs for liquid waste. The system is direct plumbed for water and liquid waste. Blank The system fluids drawer is located in the bottom center of the system. IM Acid 28 days IM Base 28 days IM Cleaner 7 days IM Wash 28 days IM Wash 28 days Onboard stability Blank This drawer is used to replace the acid, base, wash, and cleaner bottles on the system. The acid, base, and cleaner bottles each have a volume of 1500 mL. The acid and base are used to generate the chemiluminescent reaction. The cleaner is used during the daily cleaning procedure. There are 2 wash bottles, which each have a volume of 3000 mL. The wash fluid is used by the wash ring. The acid, base, and wash have an onboard stability of 28 days. The cleaner has an onboard stability of 7 days. The onboard stability of all the bottles is tracked by the system. Blank The solid waste area is located on the bottom left side of the system. Blank The cuvette and sample tip solid waste drawer is located to the right in the solid waste area. Blank The cuvette and sample tip solid waste drawer can be pulled out of the system to access the cuvette waste bin and the sample tip waste bin. The capacity of the cuvette waste is 1500 cuvettes, and the capacity of the tip waste is 1800 tips. Blank The tip tray waste is located to the left of the solid waste drawer. Blank When all of the sample tips have been used from a tray, the empty tip tray is discarded to the tip tray waste. The capacity of the tip tray waste is 15 trays. Blank The system covers are controlled by a system of interlocks. To open the front top cover, the operator must release the interlocks by selecting an unlock button in the software. Blank Let’s take a look at the components inside the system that can be accessed when the front top cover is opened. The sample tip loader is located on the left side of the system. Maximum of 1800 samples tips onboard Blank Sample tips are loaded into the sample tip loader in packs of 3. A maximum of 5 sample tip packs can be onboard on the system. Each rack contains 120 sample tips, therefore a total of 1800 sample tips can be onboard the system at a time. Blank The wash ring is located in the center of the system. Blank The wash ring is a circular track that washes and prepares reaction cuvettes for the final chemiluminescent reaction. There are four aspirate probes that remove the wash fluid from the cuvettes. After washing, the acid probe dispenses acid reagent into the cuvette. Blank The luminometer is located above the wash ring, to the right. Blank The luminometer contains the photomultiplier tube (PMT) with photon-counting electronics, base probe, and waste probe. The base probe is located under the cover of the luminometer. In this area of the system, base reagent is dispensed into the cuvette and the chemiluminescent reaction occurs. The luminometer measures the light emitted as relative light units (RLUs). The waste is aspirated and the cuvette is discarded. The system software converts the RLUs to results. Blank The cuvette bin is located to the right of the luminometer. Maximum of 1500 cuvettes onboard Blank The cuvette bin can hold a maximum of 1500 cuvettes. Blank The reagent drawer is located on the right side of the system. Ability to load up to 5 reagent packs at a time Blank Five packs of either primary or ancillary reagent can be loaded at a time using the reagent drawer. When packs are loaded into the reagent drawer, the system scans the pack barcodes and a robotic arm loads them into the reagent compartment. Blank There are additional internal components which can be viewed from the back of the system. To open the back top cover, the operator must release the cover interlocks by selecting an unlock button in the software. Blank Let’s take a look at the components inside the system that can be accessed when the back top cover is opened. The reagent compartment is located on the left side of the system, viewed from the back. Blank The reagent compartment stores both primary and ancillary reagent packs in a single refrigerated tray. The temperature of the reagent compartment is maintained at 4 to 8 degrees Celsius. The rotary tray motion maintains solid phase particle suspension. The compartment can hold up to 42 primary reagent packs and 35 ancillary reagent packs. The primary reagent contains paramagnetic particles (PMPs) and Acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the Acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test. Blank The reagent loader scans new reagent packs which the operator has loaded in the reagent drawer, and then transports the packs from the reagent drawer to the reagent compartment. Blank There are 3 reagent probes which are located above the reagent compartment, at the right edge of the reagent compartment. The reagent probes deliver primary and ancillary reagent to the cuvettes. The probes are washed after each dispense. Blank The incubation ring is located in the center of the system. Blank The incubation ring is a circular, insulated track comprised of 2 independently movable rings that advance the cuvettes at 8-second intervals to the sample and reagent positions. The incubation ring is maintained at 37 degrees Celsius. The sample probe is located on the right edge of the incubation ring. Blank The sample probe picks up a new tip from the sample tip loader for each test that is processed. After the sample is dispensed into a cuvette, the sample probe ejects the used tip into the solid waste bin. Blank Also in the back of the system, there is one fan to cool the system. The fan has a filter in front of it, located in the center rear panel. Finally, the main power switch is located in the left rear panel of the analyzer. Congratulations! You have successfully completed the System Components Overview for the Atellica IM Analyzer. To watch the simulation again select Replay, or continue by selecting Next. Replay unassigned Next Completion Question Bank 1 HOOD05162003360234 | Effective Date: 20 JUN 2023 1.1 Welcome 1.2 Hardware Components 3.1 System Components 1.3 Test Processing Sequence 1.4 Course Review 1.5 Disclaimer 1.6 Assessment