Atellica® CI Analyzer Hardware Overview Online Training

Continue Button Continue Continue Continue Continue Untitled Scene Master Template HILS2218 | Effective Date: 25-Mar-2022 ? Atellica® CI Analyzer Hardware Overview Online Training This course includes an overview of the hardware components and their function and the test processing sequences. Identify hardware components and their function 1 Describe the CH photometry test processing sequence 3 Describe the IM test processing sequence 2 Describe the CH IMT test processing sequence 4 Welcome Welcome to the Atellica CI Analyzer Hardware Overview Online Training Course. Upon successful completion of this course, you will be able to identify the hardware components and their function and describe the test processing sequence for both IM and CH tests. ? Supply and Reagent Access Select the markers on the left to view the access doors that the operator can use to access supplies, even while the system is running. IM Sample Tip Drawers IM Primary Reagent Compartment IM Solid Waste and CH Lamp Coolant Door System Fluids Drawer CH Reagent Drawer CH IMT Fluids and Onboard Water Drawer IM Ancillary Reagent Compartment IM Cuvette Loading Bin Supply and Reagent Access The top system cover must be closed for the system to process samples. There are several access doors that the operator can open to access supplies and reagents, even while the system is running. Select the markers to learn more about these access doors. ? Top Covers Select the Play button below to learn about the internal components that can be accessed by opening the top covers. Play Top Covers To open the left front cover and right front cover, the operator must first select an Unlock button in the software. Select the Play button to learn about the components inside the system that can be accessed when the top covers are opened. ? Back Covers Select the Play button below to learn about the internal components that can be accessed by removing the back covers. Play Back Covers On the back of the analyzer, there is a left back cover and a right back cover. To remove these covers, the operator must first select an Unlock button in the software. Select the Play button to learn about the components inside the system that can be accessed when the back covers are removed. ? IM Test Processing Sequence By following the journey that cuvettes take from beginning to end as a test is processed, you will gain a better perspective on how the different parts of the system function. Select the numbered steps below to learn more about the IM Test Processing Sequence. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 8 8 8 IM Test Processing Notes: By following the journey that cuvettes take from beginning to end as a test is processed, you will gain a better perspective on how the different parts of the system function. Select the numbered steps to learn more about the IM Test Processing Sequence. Notes: The first step of the test processing sequence is the transfer of cuvettes from the cuvette bin to the incubation ring. The IM cuvette loader transfers cuvettes into empty slots in the incubation ring and holds them for a designated warming up time before use. Notes: When a test begins processing, the sample probe first picks up a disposable tip from the sample tip tray. The sample probe then aspirates sample from the sample tube and transfers the sample into the cuvette in the incubation ring. After dispensing, the tip is removed from the sample probe. The tip falls down the tip waste chute into the tip solid waste bin. Notes: The reagent probe then dispenses primary and ancillary reagent into the cuvette. The primary reagent contains paramagnetic particles (PMPs) and acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test. Notes: The cuvette incubates in the incubation ring for a time specified by the assay test. During incubation, the analyte being measured in the sample and the acridinium ester label are bound to the PMPs. Notes: Following incubation, the cuvettes are elevated to the wash ring. The unbound sample and reagent are washed away from the PMPs in the cuvette in the wash ring. The wash fluid is dispensed into the cuvette through the dispense ports. Magnets positioned along the wash ring pull the PMPs to the side of the cuvette. Aspirate probes then draw fluid out of the cuvette leaving the PMPs with the bound material behind. After the final wash, fluid is aspirated out of the cuvette, acid reagent is added and the cuvette is elevated into the luminometer. Notes: The luminometer contains a photomultiplier tube (PMT) with photon-counting electronics, a base probe, and a waste probe. The base probe is located beneath the luminometer cover. When the cuvette enters the luminometer, base reagent is dispensed into the cuvette. The addition of the base to the cuvette changes the chemical environment to cause the chemiluminescent reaction to occur as the Acridinium ester oxidizes. Notes: The PMT takes the chemiluminescent measurement by measuring the light emitted as relative light units (RLUs). The system software converts the RLU raw signal into test results. Notes: Following the reaction, the liquid waste is aspirated from the cuvette by the waste probe. The cuvette is then discarded down the cuvette waste chute into the cuvette solid waste bin. Liquid waste is removed and cuvette is discarded Following the reaction, the liquid waste is aspirated from the cuvette by the waste probe. The cuvette is then discarded down the cuvette waste chute into the cuvette solid waste bin. 8 The PMT takes the measurement The PMT takes the chemiluminescent measurement by measuring the light emitted as relative light units (RLUs). The system software converts the RLU raw signal into test results. 7 Base is dispensed in the luminometer The luminometer contains a photomultiplier tube (PMT) with photon-counting electronics, a base probe, and a waste probe. The base probe is located beneath the luminometer cover. When the cuvette enters the luminometer, base reagent is dispensed into the cuvette. The addition of the base to the cuvette changes the chemical environment to cause the chemiluminescent reaction to occur as the acridinium ester oxidizes. 6 The PMPs in the cuvette are washed Following incubation, the cuvettes are elevated to the wash ring. The unbound sample and reagent are washed away from the PMPs in the cuvette in the wash ring. The wash fluid is dispensed into the cuvette through the dispense ports. Magnets positioned along the wash ring pull the PMPs to the side of the cuvette. Aspirate probes then draw fluid out of the cuvette leaving the PMPs with the bound material behind. After the final wash, fluid is aspirated out of the cuvette, acid reagent is added and the cuvette is elevated into the luminometer. 5 The cuvette incubates in the incubation ring The cuvette incubates in the incubation ring for a time specified by the assay test. During incubation, the analyte being measured in the sample and the acridinium ester label are bound to the PMPs. 4 Reagent is transferred into the cuvette The reagent probe then dispenses primary and ancillary reagent into the cuvette. The primary reagent contains paramagnetic particles (PMPs) and acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test. 3 Sample is transferred into the cuvette When a test begins processing, the sample probe first picks up a tip from the sample tip tray. The sample probe then aspirates sample from the sample tube and transfers the sample into the cuvette in the incubation ring. After dispensing, the tip is removed from the sample probe. The tip falls down the tip waste chute into the tip solid waste bin. 2 Cuvettes are transferred to the incubation ring The IM cuvette loader transfers a cuvettes from the cuvette loading bin into empty slots in the incubation ring and holds them for a designated warming up time before use. 1 ? CH Photometry Test Processing Sequence By following the journey that a sample takes as a test is processed, you will gain a better perspective on how the different parts of the system function. Select the numbered steps below to learn more about the CH Photometry Test Processing Sequence. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 CH Photometry Test Processing Notes: Now let’s take a look at another type of test that can be processed on the CI analyzer. Select the numbered steps to learn more about the CH Photometry Test Processing Sequence. Notes: When a test begins processing, the dilution probe aspirates a sample from a sample tube presented by the analyzer. The dilution probe then dispenses and dilutes the sample into a dilution cuvette on the dilution ring. The dilution mixer then stirs the diluted sample. Notes: The reagent probe then aspirates and dispenses reagent into a reaction cuvette on the reaction ring. Notes: The sample probe aspirates and dispenses the required amount of diluted sample into the reaction cuvette containing the first reagent. The remaining diluted sample in the dilution cuvette can be used for additional tests on an order. The reagent and sample mixer mixes both the reagent and the diluted sample. Notes: The reagent probe aspirates and dispenses the second reagent from the reagent compartment into the reaction cuvette containing the first reagent and the diluted sample. The reagent and sample mixer mixes the diluted sample and both reagents. Notes: The reaction takes place for the amount of time designated for the assay. After the reaction is complete, the photometer reads the light absorbance of the sample. These absorbances are then converted to qualitative, quantitative and semi-quantitative test results. Photometer takes the measurement The reaction takes place for the amount of time designated for the assay. After the reaction is complete, the photometer reads the light absorbance of the sample. These absorbances are then converted to qualitative, quantitative and semi-quantitative test results. 5 The second reagent is added to the reaction cuvette The reagent probe aspirates and dispenses the second reagent from the reagent compartment into the reaction cuvette containing the first reagent and the diluted sample. The reagent and sample mixer mixes the diluted sample and both reagents. 4 Diluted sample is transferred into the reaction cuvette The sample probe aspirates and dispenses the required amount of diluted sample into the reaction cuvette containing the first reagent. The remaining diluted sample in the dilution cuvette can be used for additional tests on an order. The reagent and sample mixer mixes both the reagent and the diluted sample. 3 The first reagent is transferred into a reaction cuvette The reagent probe then aspirates and dispenses the first reagent into a reaction cuvette on the reaction ring. 2 Diluted sample is transferred into a dilution cuvette When a test begins processing, the dilution probe aspirates a sample from a sample tube presented by the analyzer. The dilution probe then dispenses and dilutes the sample into a dilution cuvette on the dilution ring. The dilution mixer then stirs the diluted sample. 1 ? IMT Test Processing Sequence The analyzer uses integrated multisensor technology (IMT) to determine the concentration of sodium, potassium and chloride ions in patient samples. The IMT cartridge encloses a reference electrode and 3 ion-selective electrodes. Select the numbered steps below to learn more about the IMT Test Processing Sequence. 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 IMT Test Processing Notes: Next let’s look at another type of test. The analyzer uses integrated multisensor technology (IMT) to determine the concentration of sodium, potassium and chloride ions in patient samples. The IMT cartridge encloses a reference electrode and 3 ion-selective electrodes. Select the numbered steps to learn more about the IMT Test Processing Sequence. Notes: To start processing a test, Standard A solution is first pulled through the IMT cartridge. The system measures the potential difference between each of the 3 analytes in the Standard A solution and the reference electrode. Air and Standard A fluidic segments are pulled into the IMT cartridge to both clean and prepare the sensor chip for sample measurement. Notes: Next, 75 microliters of IMT diluent is pulled from the IMT port. Notes: The dilution probe aspirates 25 microliters of sample from a sample tube or cup presented by the analyzer and dispenses the sample and then 150 microliters of water into the IMT port. As the dilution probe dispenses the sample, it mixes the sample, diluent and water. The dilution probe returns to its rest position and is rinsed and cleaned. Notes: The mixed sample is pulled through the IMT cartridge. The drift correction completes when the analyzer pulls a second Standard A sequence through the IMT cartridge, comparing this reading to the initial Standard A measurement. Notes: The system measures the potential difference between each of the 3 analytes in the sample and the reference electrode. The system uses an algorithm to convert the potential differences into ion concentrations and reports this value. Measurement The system measures the potential difference between each of the 3 analytes in the sample and the reference electrode. The system uses an algorithm to convert the potential differences into ion concentrations and reports this value. 5 Mixed sample travels through the IMT cartridge The mixed sample is pulled through the IMT cartridge. The drift correction completes when the analyzer pulls a second Standard A sequence through the IMT cartridge, comparing this reading to the initial Standard A measurement. 4 Dilution probe transfers sample to the IMT port The dilution probe aspirates 25µL of sample from a sample tube or cup presented by the analyzer and dispenses the sample and then 150µL of water into the IMT port. As the dilution probe dispenses the sample, it mixes the sample, diluent and water. The dilution probe returns to its rest position and is rinsed and cleaned. 3 Prepare for measurement 75µL of IMT diluent is pulled from the IMT port. 2 Standard A solution pulled through IMT cartridge To start processing a test, Standard A solution is first pulled through the IMT cartridge. The system measures the potential difference between each of the 3 analytes in the Standard A solution and the reference electrode. Air and Standard A fluidic segments are pulled into the IMT cartridge to both clean and prepare the sensor chip for sample measurement. 1 Describe the CH photometry test processing sequence Describe the IM test processing sequence Identify hardware components and their function Course Review Congratulations. You have completed the Atellica® CI Analyzer Hardware Overview Online Training course. Select the objectives listed below to review the material before proceeding to the final assessment. 1 1 1 2 2 2 3 3 3 Describe the IMT test processing sequence 4 Course Review Describe the IMT Test Processing Sequence Standard A solution is pulled through the IMT cartridge The potential difference is measured between the analytes in Standard A solution and the reference electrode Air and fluidic segments of Standard A are pulled into the IMT cartridge to both clean and prepare the sensor chip for sample measurement The IMT diluent pump dispenses 75 µL of IMT diluent into the IMT sample port The dilution probe aspirates 25 µL of sample from a sample container The dilution probe dispenses the sample and 150 µL of special reagent water into the IMT port The dilution probe returns to its wash station for rinsing and cleaning The analyzer pulls the mixed sample through the IMT cartridge The system measures the potential difference between each of the three analytes in the sample and the reference electrode The drift correction completes when the analyzer pulls a second Standard A sequence though the IMT cartridge, comparing this reading to the reading in step 2 The system converts the potential differences into ion concentrations and reports this value Describe the CH Photometry Test Processing Sequence Diluted sample is transferred into a dilution cuvette on the dilution ring, and then mixed The first reagent is transferred into a reaction cuvette on the reaction ring An aliquot of diluted sample is transferred into the reaction cuvette containing the first reagent. The remaining diluted sample in the dilution cuvette can be used for additional tests on an order The first reagent and diluted sample are mixed The second reagent is transferred into the reaction cuvette containing the first reagent and the diluted sample The diluted sample and both reagents are mixed The reaction takes place for the amount of time designated for the assay The absorbance data is read by the photometer Describe the IM test processing sequence Cuvettes are transferred from the cuvette bin to the incubation ring Sample is transferred into the cuvette Primary and ancillary reagent are transferred into the cuvette The cuvette incubates in the incubation ring The paramagnetic particles (PMPs) in the cuvette are washed in the wash ring Acid and base are dispensed into the cuvette The photomultiplier tube (PMT) takes the chemiluminescent measurement Liquid waste is removed from the cuvette The cuvette falls into the cuvette solid waste bin Identify hardware components and their function Access doors that can be opened to access supplies and reagents, even while the system is running: IM Cuvette Loading Bin IM Sample Tip Drawers IM Primary Reagent Compartment IM Primary Reagent Compartment CH Reagent Drawer IM Ancillary Reagent Compartment CH IMT Fluids and Onboard Water Drawer System Fluids Drawer IM Solid Waste CH Lamp Coolant Door Hardware components that can be accessed by opening the front covers: IM sample probe IM sample tip drawers IMT system Pretreatment pack IM primary reagent compartment CH reagent drawer IM ancillary reagent compartment CH reagent loader IM reagent probe IM cuvette loader Hardware components that can be accessed by removing the back covers: CH dilution ring CH dilution probe CH dilution mixer CH dilution wash station CH sample probe CH reaction ring CH reaction wash station CH reaction mixer CH reagent compartment CH reagent probe IM aspirate probes IM wash ring IM luminometer Disclaimer Please note that the learning material is for training purposes only. For the proper use of the software or hardware, please always use the Operator Manual or Instructions for Use (hereinafter collectively “Operator Manual”) issued by Siemens Healthineers. This material is to be used as training material only and shall by no means substitute the Operator Manual. Any material used in this training will not be updated on a regular basis and does not necessarily reflect the latest version of the software and hardware available at the time of the training. The Operator Manual shall be used as your main reference, in particular for relevant safety information like warnings and cautions. Please note: Some functions shown in this material are optional and might not be part of your system. Certain products, product related claims or functionalities (hereinafter collectively “Functionality”) may not (yet) be commercially available in your country. Due to regulatory requirements, the future availability of said Functionalities in any specific country is not guaranteed. Please contact your local Siemens Healthineers sales representative for the most current information. The reproduction, transmission or distribution of this training or its contents is not permitted without express written authority. Offenders will be liable for damages. All names and data of patients, parameters and configuration dependent designations are fictional and examples only. All rights, including rights created by patent grant or registration of a utility model or design, are reserved. Atellica, and all associated marks are trademarks of Siemens Healthcare Diagnostics Inc. or its affiliates. All other trademarks and brands are the property of their respective owners. © Siemens Healthcare GmbH 2022 Siemens Healthineers Headquarters\Siemens Healthcare GmbH\Henkestr. 127\ 91052 Erlangen, Germany\Telephone: +49 9131 84-0\siemens-healthineers.com Disclaimer Assessment Start ? This assessment will test your retention of the presented content. A passing score of 80% or higher is required to complete the course and earn your certificate . Assessment questions must be answered completely to receive full credit. Partial credit will not be given for assessment questions that require multiple answers. You may repeat the assessment as many times as needed. Assessment Select the correct designated area, then select Submit below. Question 1 of 10 Where are the system fluids located? Hotspot Incorrect Incorrect Correct Incorrect Incorrect You did not select a designated area. Select the correct designated area, then select Submit below. Question 2 of 10 Where is the IM primary reagent compartment located? Hotspot Incorrect Incorrect Incorrect Correct Incorrect You did not select a designated area. Select the best answer. ? Question 3 of 10 Which of the following is the function of the component highlighted in this CI Analyzer image? Transfer diluted sample from the CH dilution ring into the CH reaction ring Transfer sample from the sample tube into the CH dilution cuvette Transfer reagent from the CH reagent compartment into the CH reaction ring Transfer reagent packs to the CH reagent compartment Multiple Choice Incorrect The CH reagent probe transfers reagent from the CH reagent compartment into the CH reaction ring. Incorrect The CH reagent loader transfers reagent packs to the CH reagent compartment. Incorrect The CH dilution probe transfers sample from the sample tube into the CH dilution cuvette. Correct Select the best answer. ? Question 4 of 10 Which of the following is the function of the component highlighted in this CI Analyzer image? Provides the light source for the CH photometric measurement Measures the IMT potential difference Takes the IM chemiluminescent measurement Multiple Choice Incorrect The PMT takes the IM chemiluminescent measurement. Incorrect The A-LYTE multisensor measures the IMT potential difference. Correct Select the best answer. ? Question 5 of 10 What components are found within the IM luminometer on the CI analyzer? Photomultiplier tube, base probe and waste probe Photomultiplier tube, acid probe and waste probe Photomultiplier tube, acid probe and base probe Multiple Choice Incorrect The acid probe is part of the wash ring assembly. Incorrect The acid probe is part of the wash ring assembly. Correct Select the best answer. ? CH Sample Probe Question 6 of 10 Which probe on the CI analyzer uses disposable tips? IM Sample Probe IM Reagent Probe CH Reagent Probe Multiple Choice Incorrect Incorrect Incorrect Correct Select the best answer. ? Question 7 of 10 Where is the power switch located on the CI Analyzer? On the back of the analyzer, beneath the back covers On the right side of the analyzer On the front of the analyzer, beneath the monitor Multiple Choice Incorrect Incorrect Correct Sample is transferred into the cuvette Primary and ancillary reagent are transferred into the cuvette The cuvette incubates in the incubation ring The PMPs in the cuvette are washed The PMT takes the chemiluminescent measurement What is the correct order of steps in the IM test processing sequence? Drag each response to place the list in the correct order. ? Question 8 of 10 Sequence Incorrect One or more answers are incorrect. Correct Diluted sample is transferred to the dilution ring The first reagent is transferred to the reaction ring An aliquot of diluted sample is transferred to the reaction ring The second reagent is transferred to the reaction ring The absorbance data is read by the photometer What is the correct order of steps in the CH photometry test processing sequence? Drag each response to place the list in the correct order. ? Question 9 of 10 Sequence Incorrect One or more answers are incorrect. Correct Standard A is pulled through the IMT cartridge IMT diluent is dispensed into the IMT sample port Sample and special reagent water are dispensed into the IMT sample port The potential difference between the analytes in the sample and the reference electrode is measured What is the correct order of steps in the IMT test processing sequence? Drag each response to place the list in the correct order. ? Question 10 of 10 Sequence Incorrect One or more answers are incorrect. Correct Assessment Results YOUR SCORE: PASSING SCORE: Review Retry Retry Continue Continue Continue %Results.ScorePercent%% %Results.PassPercent%% ? Assessment Results You did not pass the course. Take time to review the assessment then select Retry to continue. Congratulations. You passed the course.. Exit To access your Certificate of Completion, select the Launch button drop down on the course overview page. You can also access the certificate from your PEPconnect transcript. ? You have completed the Atellica® CI Analyzer Hardware Overview Online Training. Completion Navigation Help Select the icon above to open the table of contents. Click Next to continue. Next Welcome Slide The timeline displays the slide progression. Slide the orange bar backwards to rewind the timeline. Click Next to continue. Next Tmeline Select the CC icon to display closed captioning (subtitles). Click Next to continue. Next Caption Icon Select the buttons to learn more about a topic. Be sure to review all topics before navigating to the next slide. Click Next to continue. Next Tab Arrow Slide Select the X to close the pop-up. Click Next to continue. Next Layer Slide Some images may have a magnifier icon. Select the image to see an enlarged view. Select it again to return to the normal view. Click Next to continue. Next Zoom Slide Some images have a magnifier icon in the bottom-left corner. Select these image to see an enlarged view of the image. Select the image again to return to the normal view. Select Submit to record your response. Click the X in the upper right corner to exit the navigation help. Assessment Slide Top Covers Untitled Slide Let’s first look at the hardware components that can be accessed by opening the left front cover. Untitled Slide The IM sample probe picks up a new tip from the sample tip drawers for each IM test that is processed. After the sample is dispensed into a cuvette, the sample probe ejects the used tip into the tip waste bin. Untitled Slide The IM sample tip drawers contain the trays of sample tips. Each tray contains 120 sample tips. Two trays can be loaded to be used by the system, in the back 2 positions. The front 2 positions can be used for storing additional trays that will be loaded into the back slots by the operator at a later time. Untitled Slide The IMT system is also located in this area. The IMT system uses potentiometry to determine the concentration of sodium, potassium and chloride ions in patient samples. Here is where the A-LYTE multisensor and IMT peristaltic pump are located. Untitled Slide The pretreatment pack is located behind the IMT system. The pretreatment pack is needed for the A1C assay. Untitled Slide Now let’s move to the right side of the system and look at the hardware components that can be accessed by opening the right front cover. Untitled Slide At the front is the IM primary reagent compartment, CH reagent drawer, and IM ancillary reagent compartment. The IM primary reagent compartment and IM ancillary reagent compartment each have 20 positions for reagent packs. The primary reagent contains paramagnetic particles (PMPs) and Acridinium ester. The PMPs provide the solid phase of the chemiluminescent reaction, and the acridinium ester provides the label that is used to determine the quantity of analyte in the sample. The ancillary reagents include diluents, pretreatments, and special washes. Ancillary reagents are not required for every test. The CH reagent drawer is used to load and unload CH reagent packs. There are 4 reagent positions in the drawer for 4 reagent packs to be loaded one at a time. Untitled Slide The CH reagent loader picks up reagent packs from the CH reagent drawer and places them into the proper position in the CH reagent compartment. The loader also removes old reagent packs from the compartment and places them in the CH reagent drawer to be discarded. Untitled Slide Behind the IM ancillary reagent compartment you will find the IM reagent probe. The IM reagent probe delivers primary and ancillary reagent to the cuvettes. The probe is washed after each dispense. Untitled Slide All the way to the right is the IM cuvette loader, which is where cuvettes are loaded onto the system. Untitled Slide This completes our overview of the hardware components that can be accessed by opening the front covers. Please click on the X in the upper right-hand corner to go back to the training. Back Covers Untitled Slide Let’s first look at the hardware components that can be accessed by removing the right back cover. Untitled Slide The CH dilution ring, probe, mixer, and wash station are located on the right side. The dilution ring holds the dilution cuvettes which house the diluted samples. The dilution probe aspirates a sample from a sample tube and then dispenses and dilutes the sample into a dilution cuvette on the dilution ring. The dilution probe also transfers sample from a sample tube to the IMT system for sodium, potassium, and chloride testing. The dilution probe has clot detection, crash protection, and liquid surface verification. The dilution mixer stirs the diluted sample that has been transferred to the dilution cuvette. The diluted sample is held for retest or reflex until that cuvette reaches the dilution wash station. The dilution wash station cleans the dilution cuvette after a sample is processed, allowing the cuvettes to be reused. Untitled Slide The CH sample probe is located in between the reaction ring and the dilution ring. The CH sample probe aspirates and dispenses the required amount of diluted sample from the dilution ring into the reaction cuvette containing the first reagent. The sample probe has clot detection, crash protection, and liquid surface verification. Untitled Slide The CH reaction ring, reaction wash station, and reagent and sample mixer are located to the left of the dilution ring. The reaction ring processes photometric assays in reaction cuvettes. The reaction wash station cleans the reaction cuvettes after a sample has been processed, allowing the cuvettes to be reused. The reagent and sample mixer mixes the diluted sample and reagent. Untitled Slide The CH reagent compartment is refrigerated and contains reagents as well as the cleaners that the Atellica CI Analyzer uses for daily washing and preventing contamination. There are positions for 70 packs in the CH reagent compartment. Two positions are used for reagent probe cleaners: 1 pack each of the RPC1 and RPC2 probe cleaners. One pack of RPC4 and WBA are also required for daily system cleans. Untitled Slide The CH reagent probe aspirates reagent from the reagent compartment and dispenses the reagent into the reaction cuvettes for analysis. Untitled Slide The photometer consists of a source lamp and a detection assembly, which are mounted across from each other on the reaction ring. The source lamp provides the light for the photometric measurement. The photometer measures the light absorbance of samples as they pass in between the light source and the detection assembly. These absorbances are then converted to results that are reported on the system. Untitled Slide Now let’s look at the hardware components that can be accessed by removing the left back cover. Untitled Slide The 2 IM aspirate probes and the wash ring are located here. The aspirate probes remove wash fluid from the cuvettes as part of the paramagnetic particle (or PMP) wash process. The acid probe is part of the wash ring assembly. Untitled Slide The luminometer contains the photomultiplier tube (PMT) with photon-counting electronics, the base probe and the probe for the waste. The base probe, is located under the cover of the luminometer. In this area of the system, base reagent is dispensed into the cuvette and the chemiluminescent reaction occurs. The luminometer measures the light emitted as relative light units (RLUs). The waste is aspirated and the cuvette is discarded. The system software converts the RLUs to results. Untitled Slide Now let’s look at one more component on the back of the system – the power switch, which is located on the bottom back of the system. The power switch can be accessed without removing covers. Untitled Slide This completes our overview of the hardware components that can be accessed from the back of the system. Please click on the X in the upper right-hand corner to go back to the training. Question Bank 1 HOOD05162003283950 | Effective Date: 05-JUL-2022 1.1 Welcome 1.2 Supply and Reagent Access 1.3 Top Covers 1.4 Back Covers 1.5 IM Test Processing 1.6 CH Photometry Test Processing 1.7 IMT Test Processing 1.8 Course Review 1.9 Disclaimer 1.10 Assessment